Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Genoprotective properties and metabolites of β-Glucan-Rich edible mushrooms following their In vitro fermentation by human faecal microbiota
Δημιουργός/Συγγραφέας:	Boulaka, Athina Christodoulou, Paraschos Vlassopoulou, Marigoula Koutrotsios, Georgios Bekiaris, Georgios Zervakis, Georgios I Mitsou, Evdokia K Saxami, Georgia Kyriacou, Adamantini [EL] Ζερβού, Μαρία[EN] Zervou, Maria [EL] Γεωργιάδης, Παναγιώτης[EN] Georgiadis, Panagiotis [EL] Πλέτσα, Βασιλική[EN] Pletsa, Vassiliki
Ημερομηνία:	2020-08-04
Γλώσσα:	Αγγλικά
DOI:	10.3390/molecules25153554
Άλλο:	32759726
Περίληψη:	A variety of bioactive compounds, constituents of edible mushrooms, in particular β-glucans, i.e., a group of β-d-glucose polysaccharides abundant in the fungal cell walls, have been linked to immunomodulating, anticancer and prebiotic activities. The aim of the study was the investigation of the genoprotective effects of edible mushrooms produced by Pleurotus eryngii, Pleurotus ostreatus and Cyclocybe cylindracea (Basidiomycota). Mushrooms from selected strains of the species mentioned above were fermented in vitro using faecal inocula from healthy volunteers. The cytotoxic and anti-genotoxic properties of the fermentation supernatants (FSs) were investigated in Caco-2 human colon adenocarcinoma cells. The FSs were cytotoxic in a dose-dependent manner. Non-cytotoxic concentrations were used for the genotoxicity studies, which revealed that mushrooms' FSs have the ability to protect Caco-2 cells against tert-butyl hydroperoxide (t-BOOH), a known genotoxic agent. Their global metabolic profiling was assessed by 1H-NMR spectroscopy. A total of 37 metabolites were identified with the use of two-dimensional (2D) homo- and hetero-nuclear NMR experiments. Multivariate data analysis monitored the metabolic variability of gut microbiota and probed to biomarkers potentially associated with the health-promoting effects of edible mushrooms.
Τίτλος πηγής δημοσίευσης:	Molecules (Basel, Switzerland)
Τόμος/Κεφάλαιο:	25
Τεύχος:	15
Σελίδες:	3554
Θεματική Κατηγορία:	[EL] Βιοτεχνολογία[EN] Biotechnology [EL] Χημική Βιολογία[EN] Chemical Biology [EL] Βιοχημεία[EN] Biochemistry [EL] Μικροβιολογία[EN] Microbiology [EL] Κυτταρολογία[EN] Cytology [EL] Φασματοσκοπία[EN] Spectroscopy
Λέξεις-Κλειδιά:	NMR-based metabolomics Edible mushrooms Faecal microbiota Genoprotection In vitro fermentation β-glucans Agaricales Biological Products Caco-2 Cells Feces Fungi Humans Magnetic Resonance Spectroscopy Metabolome Metabolomics Protective Agents beta-Glucans Fermentation
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.mdpi.com/1420-3049/25/15/3554
Ηλεκτρονική διεύθυνση περιοδικού (link) :	https://www.mdpi.com/journal/molecules
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο