Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Isoflavonoid profiling and estrogen-like activity of four genista species from the greek flora
Δημιουργός/Συγγραφέας:	Cheilari, Antigoni Vontzalidou, Argyro Makropoulou, Maria Meligova, Aggeliki K Fokialakis, Nikolas Mitakou, Sofia [EL] Αλέξης, Μιχαήλ Ν.[EN] Alexis, Michael N. Aligiannis, Nektarios
Ημερομηνία:	2020-11-24
Γλώσσα:	Αγγλικά
ISSN:	1420-3049
DOI:	10.3390/molecules25235507
Άλλο:	33255446
Περίληψη:	As part of our ongoing research on phytoestrogens, we investigated the phytochemical profile and estrogen-like activities of eight extracts from the aerial parts of four Genista species of Greek flora using estrogen-responsive cell lines. Ethyl acetate and methanolic extracts of G. acanthoclada, G. depressa,G. hassertiana, and G. millii were obtained with accelerated solvent extraction and their phytochemical profiles were compared using ultra-high-performance liquid chromatography-high-resolution mass spectrometry (uHPLC-HRMS). Fourteen isoflavonoids, previously isolated from G. halacsyi, were used as reference standards for their identification in the extracts. Thirteen isoflavonoids were detected in both extracts of G. acanthoclada and G. hassertiana, while fewer and far fewer were detected in G. millii and G. depressa, respectively. The ethyl acetate extracts of G. hassertiana and G. acanthoclada displayed 2.45- and 1.79-fold higher, respectively, estrogen-like agonist activity in Ishikawa cells compared to MCF-7 cells at pharmacologically relevant concentrations. Both these extracts, but not that of G. depressa, contained mono- and di-O-β-d-glucosides of genistein as well as the aglycone, all three of which are known to display full estrogen-like activity at lower-than-micromolar concentrations. The possibility of using preparations rich in G. hassertiana and/or G. acanthoclada extracts as a potentially safer substitute for low-dose vaginal estrogen for menopausal symptoms is discussed.
Τίτλος πηγής δημοσίευσης:	Molecules (Basel, Switzerland)
Τόμος/Κεφάλαιο:	25
Τεύχος:	23
Σελίδες:	5507
Θεματική Κατηγορία:	[EL] Φαρμακευτική χημεία[EN] Pharmaceutical chemistry [EL] Κυτταρολογία[EN] Cytology
Λέξεις-Κλειδιά:	Genista acanthoclada Genista depressa Genista hassertiana Genista millii Estrogen - like activiry Isoflavones Biomarkers Cell Proliferation Estrogens Genista Humans Isoflavones MCF-7 Cells Molecular Structure Plant Extracts
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 2020 Elsevier B.V. All rights reserved
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.sciencedirect.com/science/article/abs/pii/S0927775720308554?via%3Dihub
Ηλεκτρονική διεύθυνση περιοδικού (link) :	https://www.sciencedirect.com/journal/colloids-and-surfaces-a-physicochemical-and-engineering-aspects
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο