Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	The crystal structure of a Fusarium oxysporum feruloyl esterase that belongs to the tannase family
Δημιουργός/Συγγραφέας:	Dimarogona, Maria Topakas, Evangelos Christakopoulos, Paul [EL] Χρυσίνα, Ευαγγελία Δ.[EN] Chrysina, Evangelia D.
Ημερομηνία:	2020-06
Γλώσσα:	Αγγλικά
ISSN:	0014-5793 1873-3468
DOI:	10.1002/1873-3468.13776
Άλλο:	32297315
Περίληψη:	Feruloyl esterases are enzymes of industrial interest that catalyse the hydrolysis of the ester bond between hydroxycinnamic acids such as ferulic acid and sugars present in the plant cell wall. Although there are several structures of biochemically characterized feruloyl esterases available, the structural determinants of their substrate specificity are not yet fully understood. Here, we present the crystal structure of a feruloyl esterase from Fusarium oxysporum (FoFaeC) at 2.3 Å resolution. Similar to the two other tannase-like feruloyl esterases, FoFaeC features a large lid domain covering the active site with potential regulatory role and a disulphide bond that brings together the serine and histidine of the catalytic triad. Differences are mainly observed in the metal coordination site and the substrate binding pocket. ENZYMES: E.C.3.1.1.73. DATABASES: The sequence of FoFaeC has been deposited with UniProt with accession code A0A1D3S5H0_FUSOX and the atomic coordinates of the three-dimensional structure with Protein Data Bank, with PDB code: 6FAT.
Τίτλος πηγής δημοσίευσης:	FEBS letters
Τόμος/Κεφάλαιο:	594
Τεύχος:	11
Σελίδες:	1738-1749
Θεματική Κατηγορία:	[EL] Χημική Βιολογία[EN] Chemical Biology [EL] Βιοτεχνολογία[EN] Biotechnology [EL] Βιολογία (Γενικά)[EN] Biology (General) [EL] Δομική Βιολογία[EN] Structural Biology
Λέξεις-Κλειδιά:	Fusarium oxysporum X-ray crystallography Biocatalysis Feruloyl esterase Tannase family Amino Acid Sequence Carboxylic Ester Hydrolases Catalytic Domain Crystallography, X-Ray Databases, Protein Disulfides Fusarium Models, Molecular
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 2020 Federation of European Biochemical Societies
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://febs.onlinelibrary.wiley.com/doi/10.1002/1873-3468.13776
Ηλεκτρονική διεύθυνση περιοδικού (link) :	https://febs.onlinelibrary.wiley.com/journal/18733468
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο