Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Anti-SARS-CoV-2 antibody responses in convalescent plasma donors are increased in hospitalized patients; Subanalyses of a phase 2 clinical study
Δημιουργός/Συγγραφέας:	Terpos, Evangelos Politou, Marianna Sergentanis, Theodoros N Mentis, Andreas Rosati, Margherita [EL] Στέλλας, Δημήτρης[EN] Stellas, Dimitris Bear, Jenifer Hu, Xintao Felber, Barbara K Pappa, Vassiliki Pagoni, Maria Grouzi, Elisavet Labropoulou, Stavroula Charitaki, Ioanna Ntanasis-Stathopoulos, Ioannis Moschandreou, Dimitra Bouhla, Anthi Saridakis, Stylianos Korompoki, Eleni Giatra, Chara Bagratuni, Tina Pefanis, Angelos Papageorgiou, Sotirios Spyridonidis, Alexandros Antoniadou, Anastasia Kotanidou, Anastasia Syrigos, Konstantinos Stamoulis, Konstantinos Panayiotakopoulos, George Tsiodras, Sotirios Alexopoulos, Leonidas Dimopoulos, Meletios A Pavlakis, George N
Ημερομηνία:	2020-11-28
Γλώσσα:	Αγγλικά
ISSN:	2076-2607
DOI:	10.3390/microorganisms8121885
Άλλο:	33260775
Περίληψη:	We evaluated the antibody responses in 259 potential convalescent plasma donors for Covid-19 patients. Different assays were used: a commercial ELISA detecting antibodies against the recombinant spike protein (S1); a multiplex assay detecting total and specific antibody isotypes against three SARS-CoV-2 antigens (S1, basic nucleocapsid (N) protein and receptor-binding domain (RBD)); and an in-house ELISA detecting antibodies to complete spike, RBD and N in 60 of these donors. Neutralizing antibodies (NAb) were also evaluated in these 60 donors. Analyzed samples were collected at a median time of 62 (14-104) days from the day of first symptoms or positive PCR (for asymptomatic patients). Anti-SARS-CoV-2 antibodies were detected in 88% and 87.8% of donors using the ELISA and the multiplex assay, respectively. The multivariate analysis showed that age ≥50 years (p < 0.001) and need for hospitalization (p < 0.001) correlated with higher antibody titers, while asymptomatic status (p < 0.001) and testing >60 days after symptom onset (p = 0.001) correlated with lower titers. Interestingly, pseudotype virus-neutralizing antibodies (PsNAbs) significantly correlated with spike and with RBD antibodies by ELISA. Sera with high PsNAb also showed a strong ability to neutralize active SARS-CoV-2 virus, with hospitalized patients showing higher titers. Therefore, convalescent plasma donors can be selected based on the presence of high RBD antibody titers.
Τίτλος πηγής δημοσίευσης:	Microorganisms
Τόμος/Κεφάλαιο:	8
Τεύχος:	12
Σελίδες:	1885
Θεματική Κατηγορία:	[EL] Ιατρική (Γενικά)[EN] Medicine (General) [EL] Χημική Βιολογία[EN] Chemical Biology [EL] Ανοσολογία[EN] Immunology [EL] Βιοχημεία[EN] Biochemistry
Λέξεις-Κλειδιά:	Covid-19 SARS-CoV-2 Antibodies Convalescent plasma Novel coronavirus
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 2020 by the authors
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.mdpi.com/2076-2607/8/12/1885
Ηλεκτρονική διεύθυνση περιοδικού (link) :	https://www.mdpi.com/journal/microorganisms
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο