Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Physicochemical evaluation of insulin complexes with QPDMAEMA-b-PLMA-b-POEGMA cationic amphiphlic triblock terpolymer micelles
Δημιουργός/Συγγραφέας:	Skandalis, Athanasios Murmiliuk, Anastasiia Štěpánek, Miroslav [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios
Ημερομηνία:	2020-02-03
Γλώσσα:	Αγγλικά
DOI:	10.3390/polym12020309
Άλλο:	32028685
Περίληψη:	Herein, poly[quaternized 2-(dimethylamino)ethyl methacrylate-b-lauryl methacrylate-b-(oligo ethylene glycol)methacrylate] (QPDMAEMA-b-PLMA-b-POEGMA) cationic amphiphilic triblock terpolymers were used as vehicles for the complexation/encapsulation of insulin (INS). The terpolymers self-assemble in spherical micelles with PLMA cores and mixed QPDMAEMA/POEGMA coronas in aqueous solutions. The cationic micelles were complexed via electrostatic interactions with INS, which contains anionic charges at pH 7. The solutions were colloidally stable in all INS ratios used. Light-scattering techniques were used for investigation of the complexation ability and the size and surface charge of the terpolymer/INS complexes. The results showed that the size of the complexes increases as INS ratio increases, while at the same time the surface charge remains positive, indicating the formation of clusters of micelles/INS complexes in the solution. Fluorescence spectroscopy measurements revealed that the conformation of the protein is not affected after the complexation with the terpolymer micellar aggregates. It was observed that as the solution ionic strength increases, the size of the QPDMAEMA-b-PLMA-b-POEGMA/INS complexes initially decreases and then remains practically constant at higher ionic strength, indicating further aggregation of the complexes. atomic force microscopy (AFM) measurements showed the existence of both clusters and isolated nanoparticulate terpolymer/protein complexes.
Τίτλος πηγής δημοσίευσης:	Polymers Special Issue: Self-assembly of Block Copolymers
Τόμος/Κεφάλαιο:	12
Τεύχος:	2
Σελίδες:	309
Θεματική Κατηγορία:	[EL] Χημεία (Γενικά)[EN] Chemistry (General) [EL] Φασματοσκοπία[EN] Spectroscopy [EL] Χημική τεχνολογία[EN] Chemical technolgy
Λέξεις-Κλειδιά:	Cationic polymers Insulin nanocarriers Polyectrolyte/protein complexes Protein nanocarriers Triblock terpolymers
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.mdpi.com/2073-4360/12/2/309
Ηλεκτρονική διεύθυνση περιοδικού (link) :	https://www.mdpi.com/journal/polymers
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο