Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Amphiphilic QP(DMAEMA-co-LMA)-b-POEGMA random-block terpolymers as nanocarriers for insulin
Δημιουργός/Συγγραφέας:	Kafetzi, Martha [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios Bao, Xiaoyan Yao, Ping
Εκδότης:	MDPI
Ημερομηνία:	2020-10-04
Γλώσσα:	Αγγλικά
ISSN:	2227-9059
DOI:	10.3390/biomedicines8100392
Άλλο:	33020449
Περίληψη:	We report on the utilization of the amphiphilic poly[quaternized (2-(N,N-dimethylamino) ethyl methacrylate)]-co-(lauryl methacrylate))-b-poly[(oligo ethylene glycol) methyl ether methacrylate] QP(DMAEMA-co-LMA)-b-POEGMA cationic diblock terpolymer aggregates as nanocarriers for insulin delivery applications. QP(DMAEMA-co-LMA)-b-POEGMA random diblock terpolymer is derived from the chemical modification of the precursor amino diblock copolymer via quaternization, producing permanent positive charges on the macromolecular chain. The QP(DMAEMA-co-LMA)-b-POEGMA diblock terpolymer as well as its amino precursor investigated self-assemble in aqueous media, forming aggregates. In vitro cytotoxicity and in vivo biocompatibility studies on QP(DMAEMA-co-LMA)-b-POEGMA and its amino precursor aggregates, showed good cytocompatibility and biocompatibility. QP(DMAEMA-co-LMA)-b-POEGMA aggregates were chosen to be complexed with insulin due to their self-assembly features and the permanent positive charge in each amino group. QP(DMAEMA-co-LMA)-b-POEGMA aggregates were complexed with insulin through electrostatic interactions. Light scattering techniques were used in order to study the ability of the polymer aggregates to complex with insulin, to determine critical physicochemical parameters such as size, mass, and surface charge of the stable complexes and study the effect of salt addition on their properties. The results showed that in both cases, the complexation process was successful and as the insulin concentration increases, nanosized complexes of different physicochemical characteristics (mass, size, surface charge) and spherical morphology are formed. UV-Vis and fluorescence spectroscopy studies showed that no conformational changes of insulin occurred after the complexation.
Τίτλος πηγής δημοσίευσης:	Biomedicines Special Issue: Protein Delivery In Vitro or In Vivo
Τόμος/Κεφάλαιο:	8
Τεύχος:	10
Σελίδες:	392
Θεματική Κατηγορία:	[EL] Βιοχημεία[EN] Biochemistry [EL] Φασματοσκοπία[EN] Spectroscopy [EL] Ιατρική[EN] Medicine [EL] Χημική τεχνολογία[EN] Chemical technolgy
Λέξεις-Κλειδιά:	Electrostatic interactions In vitro cytotoxicity In vivo biocompatibility Insulin Polyelectrolytes Polymer/insulin complexes Protein delivery Protein nanocarriers Random diblock terpolymer Self-assembly
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.mdpi.com/2227-9059/8/10/392
Ηλεκτρονική διεύθυνση περιοδικού (link) :	https://www.mdpi.com/journal/biomedicines
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο