Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Nanoformulation of fibrinogen by thermal stabilization of its electrostatic complexes with hyaluronic acid
Δημιουργός/Συγγραφέας:	Vlassi, Eleni [EL] Παπαγιαννόπουλος, Αριστείδης[EN] Papagiannopoulos, Aristeidis
Εκδότης:	Elsevier
Ημερομηνία:	2020-05-01
Γλώσσα:	Αγγλικά
ISSN:	01418130
DOI:	10.1016/j.ijbiomac.2020.04.244
Άλλο:	32360964
Περίληψη:	The formulation of well-defined and stable fibrinogen-based nanoparticles (NPs) without the use of any chemical reaction or any toxic organic solvent is reported. Electrostatic interaction between hyaluronic acid (HA) and fibrinogen (Fbg) leads to well-defined complexes at acidic pH which however readily dissolve at neutral pH. On the other hand, when thermal treatment is applied on the pre-formed complexes NPs keep their integrity. Circular dichroism indicates that the protein's native secondary conformation in the final NPs is not affected by the formulation. The tendency of the complexes to aggregate at elevated ionic strengths is greatly suppressed after the application of the temperature treatment protocol. This characteristic is even more pronounced at neutral pH and it is connected to the enhanced surface charge of the NPs. The encapsulation of the hydrophobic compound curcumin causes only weak secondary aggregation. This work shows that the ability of Fbg to self-assemble upon thermal treatment can be effectively used to stabilize Fbg nanoformulations inside complexes with polysaccharides.
Τίτλος πηγής δημοσίευσης:	International journal of biological macromolecules
Τόμος/Κεφάλαιο:	158
Σελίδες:	251-257
Θεματική Κατηγορία:	[EL] Χημεία (Γενικά)[EN] Chemistry (General) [EL] Φασματοσκοπία[EN] Spectroscopy [EL] Χημική τεχνολογία[EN] Chemical technolgy
Λέξεις-Κλειδιά:	Curcumin Fibrinogen Hyaluronic acid Light scattering Polysaccharide-protein nanoparticles Thermal treatment
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 2020 Elsevier B.V. All rights reserved
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.sciencedirect.com/science/article/abs/pii/S0141813020331160?via%3Dihub
Ηλεκτρονική διεύθυνση περιοδικού (link) :	https://www.sciencedirect.com/journal/international-journal-of-biological-macromolecules
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο