Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Combining particle tracking microrheology and viscometry for the study of DNA aqueous solutions
Δημιουργός/Συγγραφέας:	Stefanopoulou, Evdokia [EL] Παπαγιαννόπουλος, Αριστείδης[EN] Papagiannopoulos, Aristeidis
Εκδότης:	Wiley
Ημερομηνία:	2020-06
Γλώσσα:	Αγγλικά
ISSN:	0006-3525 1097-0282
DOI:	10.1002/bip.23353
Άλλο:	32227481
Περίληψη:	We use video particle tracking microrheology (VPTMR) in order to investigate the viscoelasticity of salmon DNA and correlate it to its steady-flow shear-thinning viscosity. Aqueous solutions of DNA are tested in a wide concentration range from the dilute to the semidilute unentangled concentration regime. The observed mean squared displacement shows power-law scaling with lag-time which is equivalent to power-law behavior of the complex modulus as a function of frequency that is, |G* (ω)| = S ∙ ω α . The relaxation exponent α changes abruptly with concentration in the semidilute regime from about 1 to about 0.5 which is the exponent predicted by the Rouse model. The quasi-property S follows the scaling of viscosity for uncharged polymers near θ-conditions in the semidilute regime that is, η∼c1/3νeff-1 with νeff = 0.50 - 0.51. The shear-thinning exponent observed by viscometry increases gradually towards the value of 0.5 which has been predicted for Rouse chains under flow. Our findings are in agreement with recent studies of DNA solutions where DNA is treated as a model polymer and addresses the low-molar mass regime of DNA viscoelasticity. This work demonstrates that the combination of passive particle tracking with viscometry can provide a complete picture on the viscoelasticity of DNA-based biopolymer materials.
Τίτλος πηγής δημοσίευσης:	Biopolymers
Τόμος/Κεφάλαιο:	111
Τεύχος:	6
Σειρά δημοσίευσης:	e23353
Θεματική Κατηγορία:	[EL] Χημεία (Γενικά)[EN] Chemistry (General) [EL] Χημική τεχνολογία[EN] Chemical technolgy [EL] Χημική Βιολογία[EN] Chemical Biology
Λέξεις-Κλειδιά:	Particle tracking microrheology Salmon DNA Shear thinning Viscometry Animals DNA Elasticity Male Microscopy, Video Microtechnology Polymers Rheology Single Molecule Imaging Solutions Viscosity Water
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 2020 John Wiley & Sons, Inc. All rights reserved
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://onlinelibrary.wiley.com/doi/10.1002/bip.23353
Ηλεκτρονική διεύθυνση περιοδικού (link) :	https://onlinelibrary.wiley.com/journal/10970282
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο