Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Impact of chromosome 9 numerical imbalances in oral squamous cell carcinoma: A pilot grid-based centromere analysis
Δημιουργός/Συγγραφέας:	Kyrodimos, Efthymios Chrysovergis, Aristeidis Mastronikolis, Nicholas Tsiambas, Evangelos [EL] Ριζιώτης, Χρήστος Δ.[EN] Riziotis, Christos Roukas, Dimitrios Fotiades, Panagiotis Stavraka, Chara Ragos, Vasileios Paschopoulos, Minas Papanikolaou, Vasileios
Εκδότης:	MDPI
Ημερομηνία:	2020-07-21
Γλώσσα:	Αγγλικά
ISSN:	2075-4418
DOI:	10.3390/diagnostics10070501
Άλλο:	32708098
Περίληψη:	Oral squamous cell carcinoma (OSCC) is considered an aggressive malignancy, mainly due to its increased propensity to provide local and distant lymph node metastases. Gross chromosome instability (CI; polysomy/aneuploidy/monosomy), combined or not with specific gene alterations, is implicated in the development and progression of solid malignancies, including OSCC. In order to further study the relationship between these genetic alterations and the aggressive biological behavior of OSCCs, we investigated the frequency and impact of chromosome 9 numerical imbalances in these tumors. Fifty (n = 50) formalin-fixed, paraffin-embedded primary OSCC tissue sections were used. Chromogenic in situ hybridization (CISH) was implemented for detecting chromosome 9 (CEN-centromere enumeration) numerical alterations. Concerning the screening process in CISH slides, a novel, real-time reference and calibration grid platform was implemented. Chromosome 9 polysomy was observed in 8/50 (16%) tissue sections, whereas the rest of them demonstrated a normal, diploid pattern (42/50; 84%). Chromosome 9 polysomy was associated with the grade of differentiation of the examined tumors (p = 0.036). Chromosome 9 numerical imbalances (polysomy) were observed in sub-groups of OSCCs correlating with a progressive dedifferentiation of the malignant tissues. Concerning the implementation of the proposed grid-based platform as described above on CISH slides, it provides a novel, fast, and accurate screening mapping mechanism for detecting chromosome numerical imbalances.
Τίτλος πηγής δημοσίευσης:	Diagnostics (Basel, Switzerland) Special Issue: Biomarkers of Oral Cancer
Τόμος/Κεφάλαιο:	10
Τεύχος:	7
Σελίδες:	501
Θεματική Κατηγορία:	[EL] Νεοπλάσματα. Όγκοι. Ογκολογία (περ. Καρκίνος, κακινογόνες ουσίες)[EN] Neoplasms. Tumors. Oncology (Incl.cancer, carcinogens) [EL] Κυτταρολογία[EN] Cytology [EL] Γενετική[EN] Genetics [EL] Βιολογία (Γενικά)[EN] Biology (General)
Λέξεις-Κλειδιά:	Carcinoma Chromosome Grid Microscopy Oral Polysomy
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.mdpi.com/2075-4418/10/7/501
Ηλεκτρονική διεύθυνση περιοδικού (link) :	https://www.mdpi.com/journal/diagnostics
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο