Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Combined transcriptomic and phosphoproteomic analysis of BMP4 signaling in human embryonic stem cells
Δημιουργός/Συγγραφέας:	Papadopoulos, Angelos Chalmantzi, Varvara Mikhaylichenko, Olga Hyvönen, Marko [EL] Στέλλας, Δημήτρης[EN] Stellas, Dimitris Kanhere, Aditi Heath, John Cunningham, Debbie L Fotsis, Theodore Murphy, Carol
Εκδότης:	Elsevier
Ημερομηνία:	2020-12-18
Γλώσσα:	Αγγλικά
ISSN:	18735061
DOI:	10.1016/j.scr.2020.102133
Άλλο:	33383406
Περίληψη:	Human embryonic stem cells (hESCs) are an invaluable tool in the fields of embryology and regenerative medicine. Activin A and BMP4 are well-characterised growth factors implicated in pluripotency and differentiation. In the current study, hESCs are cultured in a modified version of mTeSR1, where low concentrations of ActivinA substitute for TGFβ. This culture system is further used to investigate the changes induced by BMP4 on hESCs by employing a combination of transcriptomic and phosphoproteomic approaches. Results indicate that in a pluripotent state, hESCs maintain WNT signaling under negative regulation by expressing pathway inhibitors. Initial stages of differentiation are characterized by upregulation of WNT pathway ligands, TGFβ pathway inhibitors which have been shown in Xenopus to expand the BMP signaling range essential for embryonic patterning, and mesendodermal transcripts. Moreover, BMP4 enhances the phosphorylation of proteins associated with migration and transcriptional regulation. Results further indicate the vital regulatory role of Activin A and BMP4 in crucial fate decisions in hESCs.
Τίτλος πηγής δημοσίευσης:	Stem cell research
Τόμος/Κεφάλαιο:	50
Σελίδες:	102133
Θεματική Κατηγορία:	[EL] Κυτταρολογία[EN] Cytology [EL] Βιοχημεία[EN] Biochemistry [EL] Βιολογία (Γενικά)[EN] Biology (General) [EL] Βιοπληροφορική[EN] Bioinformatics [EL] Χημική Βιολογία[EN] Chemical Biology
Λέξεις-Κλειδιά:	Activin A BMP4 Human embryonic stem cells Phosphoproteomics Transcriptomics
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 2020 The Authors. Published by Elsevier B.V.
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.sciencedirect.com/science/article/pii/S1873506120304347?via%3Dihub
Ηλεκτρονική διεύθυνση περιοδικού (link) :	https://www.sciencedirect.com/journal/stem-cell-research
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο