Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	On the identification and quantification of ergothioneine and lovastatin in various mushroom species: Assets and challenges of different analytical approaches
Δημιουργός/Συγγραφέας:	Tsiantas, Konstantinos Tsiaka, Thalia Koutrotsios, Georgios [EL] Σιάπη, Ελένη[EN] Siapi, Eleni Zervakis, Georgios I Kalogeropoulos, Nick [EL] Ζουμπουλάκης, Παναγιώτης[EN] Zoumpoulakis, Panagiotis
Εκδότης:	MDPI
Ημερομηνία:	2021-03-24
Γλώσσα:	Αγγλικά
ISSN:	1420-3049
DOI:	10.3390/molecules26071832
Άλλο:	33805096
Περίληψη:	In recent years, mushrooms have drawn the attention of agro-industries and food-industries as they were considered to be valuable natural sources of health promoting compounds such as β-glucans, ergothioneine, and lovastatin. The detection and quantification of such compounds by implementing reliable analytical approaches is of the utmost importance in order to adjust mushrooms' cultivation conditions and maximize the production in different species. Toward this direction, the current study focuses on the comparison of ultraviolet-visible (UV-Vis) spectrometry and liquid chromatography-mass spectrometry (LC-MS) methods (a) by evaluating the content of ergothioneine and lovastatin in mushrooms and (b) by highlighting any possible substrate-based interferences that hinder the accurate determination of these two compounds in order to propose the technique-of-choice for a standardized bioactive compounds monitoring. For this purpose, mushrooms produced by three species (i.e., Agaricus bisporus, Pleurotus ostreatus, and P. citrinopileatus) on various cultivation substrates, namely wheat straw (WS), winery (grape marc (GM)), and olive oil (OL) by-products, were examined. Among the two applied techniques, the developed and validated LC-MS methods, exhibiting relatively short analysis time and higher resolution, emerge as the methods-of-choice for detecting ergothioneine and lovastatin in mushrooms. On the contrary, UV-Vis methods were hindered due to co-absorbance of different constituents, resulting in invalid results. Among the studied mushrooms, P. citrinopileatus contained the highest amount of ergothioneine (822.1 ± 20.6 mg kg-1 dry sample), whereas A. bisporus contained the highest amounts of lovastatin (1.39 ± 0.014 mg kg-1 dry sample). Regarding the effect of different cultivation substrates, mushrooms produced on OL and WS contained the highest amount of ergothioneine, while mushrooms deriving from GM-based substrates contained the highest amount of lovastatin.
Τίτλος πηγής δημοσίευσης:	Molecules (Basel, Switzerland) Special Issue: Bioactive Compounds with Applications in Health and Food
Τόμος/Κεφάλαιο:	26
Τεύχος:	7
Σελίδες:	1832
Θεματική Κατηγορία:	[EL] Φασματοσκοπία[EN] Spectroscopy [EL] Χημικά προϊόντα: Παραγωγή, χρήση κλπ[EN] Chemicals: Manufacture, use etc. [EL] Μικροβιολογία[EN] Microbiology [EL] Αναλυτική χημεία[EN] Analytical chemistry [EL] Βιοτεχνολογία[EN] Biotechnology
Λέξεις-Κλειδιά:	Ergothioneine Liquid chromatography–mass spectrometry (LC–MS) Lovastatin Mushrooms Ultraviolet–visible spectroscopy (UV–Vis) Agaricus Ergothioneine Lovastatin Mycelium Pleurotus
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 2021 by the Authors. Licensee MDPI, Basel, Switzerland.
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.mdpi.com/1420-3049/26/7/1832
Ηλεκτρονική διεύθυνση περιοδικού (link) :	https://www.mdpi.com/journal/molecules
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο