Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Bromamine T, a stable active bromine compound, prevents the LPS‑induced inflammatory response
Δημιουργός/Συγγραφέας:	Baliou, Stella Sofopoulos, Michael Goulielmaki, Maria [EL] Σπαντίδος, Δημήτριος Α.[EN] Spandidos, Demetrios A. Ioannou, Petros Kyriakopoulos, Anthony M [EL] Ζουμπουρλής, Βασίλης[EN] Zoumpourlis, Vassilis
Εκδότης:	Spandidos publications
Ημερομηνία:	2021-04
Γλώσσα:	Αγγλικά
ISSN:	1107-3756 1791-244X
DOI:	10.3892/ijmm.2021.4870
Άλλο:	33537817
Περίληψη:	Inflammation is the most common cause of most acute and chronic debilitating diseases. Towards unveiling novel therapeutic options for patients with such complications, N‑bromotaurine (TauNHBr) has emerged as a potential anti‑inflammatory agent; however, its therapeutic efficacy is hindered due to its relatively poor stability. To address this challenge, the present study focused on examining the effects of a stable active bromine compound, named bromamine T (BAT). The present study examined the protective properties of BAT against lipopolysaccharide (LPS)‑mediated inflammation in vitro, by using LPS‑stimulated murine J774.A1 macrophages (Mφs), as well as in vivo, by using a murine LPS‑mediated air‑pouch model. Additionally, its efficacy was compared with that of taurine, a known potent anti‑inflammatory molecule. In LPS‑stimulated J774A.1 Mφs, BAT and taurine were very effective in reducing the secretion of pro‑inflammatory mediators. The in vitro experiments indicated that LPS‑mediated inflammation was attenuated due to the protective properties of BAT and of taurine, probably through the inhibition of phosphorylated p65 NF‑κB subunit (Ser 536) nuclear translocation. The in vivo experiments also revealed that BAT and taurine inhibited LPS‑mediated inflammation by reducing total cell/polymorphonuclear cell (PMN) infiltration in the air‑pouch and by decreasing pouch wall thickness. The analysis of exudates obtained from pouches highlighted that the inhibitory effects of BAT and taurine on the secretion of pro‑inflammatory cytokines were similar to those observed in vitro. Notably, the effect of BAT at the highest concentration tested was superior to that of taurine at the highest concentration. Taken together, the findings of the present study indicate that BAT prevents the LPS‑induced inflammatory response both in vitro and in vivo.
Τίτλος πηγής δημοσίευσης:	International journal of molecular medicine
Τόμος/Κεφάλαιο:	47
Τεύχος:	4
Σελίδες:	37
Θεματική Κατηγορία:	[EL] Ιατρική (Γενικά)[EN] Medicine (General) [EL] Χημική Βιολογία[EN] Chemical Biology [EL] Κυτταρολογία[EN] Cytology [EL] Βιοχημεία[EN] Biochemistry [EL] Θεραπευτική. Φαρμακολογία[EN] Therapeutics.Pharmacology
Λέξεις-Κλειδιά:	Bromamine T Taurine Pro-inflammatory cytokines Air-pouch model of inflammation Animals Anti-Inflammatory Agents Bromine Cell Line Cell Nucleus Disease Models, Animal Gene Expression Regulation Inflammation Inflammation Mediators Lipopolysaccharides Mice, Inbred C57BL Phosphorylation Protein Transport RNA, Messenger Sulfonamides Transcription Factor RelA Transcription, Genetic
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 2021 The Authors
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.spandidos-publications.com/10.3892/ijmm.2021.4870
Ηλεκτρονική διεύθυνση περιοδικού (link) :	https://www.https://www.spandidos-publications.com/ijmm.com/ijmm
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο