Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Three-Dimensional cell metabolomics deciphers the anti-angiogenic properties of the radioprotectant amifostine
Δημιουργός/Συγγραφέας:	[EL] Κατσίλα, Θεοδώρα[EN] Katsila, Theodora Chasapi, Styliani A Gomez Tamayo, Jose Carlos Chalikiopoulou, Constantina [EL] Σιάπη, Ελένη[EN] Siapi, Eleni Moros, Giorgos [EL] Ζουμπουλάκης, Παναγιώτης[EN] Zoumpoulakis, Panagiotis Spyroulias, Georgios A Kardamakis, Dimitrios
Εκδότης:	MDPI
Ημερομηνία:	2021-06-09
Γλώσσα:	Αγγλικά
ISSN:	2072-6694
DOI:	10.3390/cancers13122877
Άλλο:	34207535
Περίληψη:	Aberrant angiogenesis is a hallmark for cancer and inflammation, a key notion in drug repurposing efforts. To delineate the anti-angiogenic properties of amifostine in a human adult angiogenesis model via 3D cell metabolomics and upon a stimulant-specific manner, a 3D cellular angiogenesis assay that recapitulates cell physiology and drug action was coupled to untargeted metabolomics by liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy. The early events of angiogenesis upon its most prominent stimulants (vascular endothelial growth factor-A or deferoxamine) were addressed by cell sprouting measurements. Data analyses consisted of a series of supervised and unsupervised methods as well as univariate and multivariate approaches to shed light on mechanism-specific inhibitory profiles. The 3D untargeted cell metabolomes were found to grasp the early events of angiogenesis. Evident of an initial and sharp response, the metabolites identified primarily span amino acids, sphingolipids, and nucleotides. Profiles were pathway or stimulant specific. The amifostine inhibition profile was rather similar to that of sunitinib, yet distinct, considering that the latter is a kinase inhibitor. Amifostine inhibited both. The 3D cell metabolomics shed light on the anti-angiogenic effects of amifostine against VEGF-A- and deferoxamine-induced angiogenesis. Amifostine may serve as a dual radioprotective and anti-angiogenic agent in radiotherapy patients.
Τίτλος πηγής δημοσίευσης:	Cancers
Τόμος/Κεφάλαιο:	13
Τεύχος:	12
Σελίδες:	2877
Θεματική Κατηγορία:	[EL] Νεοπλάσματα. Όγκοι. Ογκολογία (περ. Καρκίνος, κακινογόνες ουσίες)[EN] Neoplasms. Tumors. Oncology (Incl.cancer, carcinogens) [EL] Χημική τεχνολογία[EN] Chemical technolgy [EL] Φασματοσκοπία[EN] Spectroscopy [EL] Φαρμακευτική χημεία[EN] Pharmaceutical chemistry
Λέξεις-Κλειδιά:	3D cellular angiogenesis assay Amifostine Anti-angiogenesis Drug repurposing Metabolomics Radioprotection
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 2021 by the authors. Licensee MDPI, Basel, Switzerland
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.mdpi.com/2072-6694/13/12/2877
Ηλεκτρονική διεύθυνση περιοδικού (link) :	https://www.mdpi.com/journal/cancers
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο