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Εξειδίκευση τύπου : Άρθρο σε επιστημονικό περιοδικό
Τίτλος: One-step separation system of bio-functional lipid compounds from natural sources
Δημιουργός/Συγγραφέας: Tsoupras, Alexandros
Pappas, Katherine M
[EL] Σωτηρούδης, Θεόδωρος Γ.[EN] Sotiroudis, Theodore G.semantics logo
Demopoulos, Constantinos A
Εκδότης: Elsevier
Ημερομηνία: 2021
Γλώσσα: Αγγλικά
ISSN: 2215-0161
DOI: 10.1016/j.mex.2021.101380
Άλλο: 34430276
Περίληψη: Lipids are a very heterogeneous class of biomolecules with distinct structures and functions. Total lipids (TLs) obtained from natural sources are regularly further separated into lipid subclasses, with the two major ones being the polar lipids (PLs) and neutral lipids (NLs). Traditional analytical methods for fractionating TLs into NLs, PLs, and their subclasses, usually comprise difficult, costly and time-consuming steps. Instead, several benefits and applications are derived by implementing a novel one-step semi-preparative and reversed-phase HPLC-analysis for separating TLs into all kinds of lipid subclasses. This method allows a one-step separation/fractionation of several subclasses of bio-functional PLs (i.e. phospholipids, glycolipids, phenolic compounds, N-acyl-homoserine-lactones, etc.) and NLs (i.e. triacylglycerols, fatty acids, esters, etc.) from TL-extracts of a natural source, prior to further testing them for their bio-functionality (i.e. in bioassays/cell models) and structure-activity relationships (i.e. LC-MS/GC-MS).•This method can be applied in several natural sources, such as animal and marine sources, plants, microorganisms of biotechnological and agricultural interest, foods, beverages and related products, and by-products.•This method can also be applied for separating specific bio-functional lipids from complex medical and pharmaceutical samples (i.e. cells, tissues, blood, plasma, liposomes, etc.), either for evaluating their role in diseases (i.e. PAF/PAF-like molecules) or by elucidating their protective roles (i.e. PLs rich in ω3 PUFA) for supplements and nutraceuticals' applications.
Τίτλος πηγής δημοσίευσης: MethodsX
Τόμος/Κεφάλαιο: 8
Σελίδες: 101380
Θεματική Κατηγορία: [EL] Γεωργία (Γενικά)[EN] Agriculture (General)semantics logo
[EL] Βιοτεχνολογία[EN] Biotechnologysemantics logo
[EL] Αναλυτική χημεία[EN] Analytical chemistrysemantics logo
Λέξεις-Κλειδιά: GC, gas chromatography
Glycolipids
HPLC
HPLC, high pressure (performance) liquid chromatography
LC, liquid chromatography
MS, mass spectra
NL(s), neutral lipid(s)
Natural origin
Neutral lipids
PAF
PAF, platelet-activating factor
PL(s), polar lipid(s)
Phenolic compounds
Phospholipids
Polar lipids
Reversed phase
TL(s), total lipid(s)
Total lipids
ω3 PUFA, omega-3 polyunsaturated fatty acids
Κάτοχος πνευματικών δικαιωμάτων: Copyright © 2021 The Authors. Published by Elsevier B.V.
Ηλεκτρονική διεύθυνση στον εκδότη (link): https://methods-x.com/article/S2215-0161(21)00173-4/fulltext
Ηλεκτρονική διεύθυνση περιοδικού (link) : https://methods-x.com/
Εμφανίζεται στις συλλογές:Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο

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