Εξειδίκευση τύπου :	Ανακοίνωση σε συνέδριο
Τίτλος:	Structural and biochemical studies of an odorant binding protein from the malaria vector anopheles gambiae
Δημιουργός/Συγγραφέας:	Liggri, P. Tsitsanou, K. [EL] Ζωγράφος, Σπύρος Ε.[EN] Zographos, Spyros E.
Εκδότης:	John Wiley & Sons, Ltd
Ημερομηνία:	2019
Γλώσσα:	Αγγλικά
ISSN:	2211-5463
DOI:	10.1002/2211-5463.12675
Περίληψη:	Anopheles gambiae is the primary mosquito vector responsible for the transmission of malaria, causing more than 1 million deaths each year. Mosquitoes rely on olfaction to find mates, food, and sources of blood meals. Odorant binding proteins (OBPs) that mediate the initial steps in the transduction cascade of olfactory signals in insects have been suggested to play an essential role in the detection and transportation of semiochemicals to odorant receptors (ORs), and thus, they constitute promising targets for the design of novel repellent/attractant molecules. Therefore, a detailed knowledge of their 3D structures and functionalities may provide a valuable tool for the structure-based discovery of novel olfactory disruptors of insect host-seeking behavior to be used in more effective mosquito control strategies. Herein, we present the novel 3D crystal structure of AgamOBP, an OBP that displays the highest levels of expression in the female antenna. These levels also appear to be affected by the circadian cycle as they dramatically reduced in constant dark (DD) conditions compared to light-dark (LD) circles. Furthermore, co-crystallization and fluorescence displacement experiments revealed the ligand-binding site of AgamOBP as well as the binding modes and specificities of various natural volatile compounds with repellent properties. This information will contribute to the better understanding of the molecular basis of odorant perception. In addition, it will guide the generation of OBP-structure-based “olfactophore” models to be used in extensive virtual screening processes for the identification of novel candidate disruptors of host-seeking mosquito behavior. Such compounds, with enhanced binding affinity and specificity for a key OBP can be used at lower concentrations and be detected over longer distances, thus providing new effective repellents for the prevention of mosquito-borne diseases.
Όνομα εκδήλωσης:	44th FEBS Congress, From Molecules to Living Systems
Ημ/νία έναρξης εκδήλωσης :	2019-07-06
Ημ/νία λήξης εκδήλωσης :	2019-07-11
Τόπος εκδήλωσης:	Krakow, Poland
Τίτλος πηγής δημοσίευσης:	FEBS Open Bio
Τόμος/Κεφάλαιο:	9
Τεύχος:	Supplement 1
Σελίδες:	269
Θεματική Κατηγορία:	[EL] Χημική Βιολογία[EN] Chemical Biology [EL] Βιοχημεία[EN] Biochemistry [EL] Δομική Βιολογία[EN] Structural Biology [EL] Φαρμακευτική[EN] Pharmacy and materia medica
Λέξεις-Κλειδιά:	Malaria Anopheles gambiae Odorant binding proteins (OBPs) Repellents mosquito-borne diseases odorant receptors (ORs) AgamOBP1
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 2019 The Authors. FEBS Open Bio
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://febs.onlinelibrary.wiley.com/doi/10.1002/2211-5463.12675
Ηλεκτρονική διεύθυνση περιοδικού (link) :	https://febs.onlinelibrary.wiley.com/journal/22115463
Σημειώσεις:	Poster: P-27-070
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο