Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Polysaccharide–Protein Multilayers Based on Chitosan–Fibrinogen Assemblies for Cardiac Cell Engineering
Δημιουργός/Συγγραφέας:	Kitsara, Maria Tassis, George [EL] Παπαγιαννόπουλος, Αριστείδης[EN] Papagiannopoulos, Aristeidis Simon, Alexandre Agbulut, Onnik [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios
Εκδότης:	John Wiley & Sons, Ltd
Ημερομηνία:	2021
Γλώσσα:	Αγγλικά
ISSN:	1616-5187 1616-5195
DOI:	10.1002/mabi.202100346
Περίληψη:	The cell and tissue culture substrates play a pivotal role in the regulation of cell-matrix and cell-cell interactions. The surface properties of the materials control a wide variety of cell functions. Amongst various methods, layer-by-layer (LbL) assembly is a versatile surface coating technique for creating controllable bio-coatings. Here, polysaccharide/protein multilayers are proposed, which are fabricated by immersive LbL assembly and based on the chitosan/fibrinogen pair for improving the adhesion and spreading of cardiomyocytes. Two approaches in LbL assembly are employed for clarifying the effect of the bilayers order and their concentration on cardiomyocytes viability and morphology. Fourier transform infrared spectroscopy (FTIR) measurements show that the adsorption of the biopolymers is enhanced during the LbL deposition in a synergistic manner. Contact angle measurements indicate that the multilayers are alternating from less to more hydrophilic behavior depending on the biopolymer that is added last. Confocal microscopy with immunostained fibrinogen reveals that the amount of the protein is higher when the concentration of the immersion solution is increased, however, for low solution concentration it is speculated that interdigitation between the separate biopolymer layers takes place. This work motivates the use of fibrinogen in polysaccharide/protein multilayers for enhanced cytocompatibility in cardiac tissue engineering.
Τίτλος πηγής δημοσίευσης:	Macromolecular Bioscience
Τόμος/Κεφάλαιο:	22
Τεύχος:	1
Σελίδες:	2100346
Θεματική Κατηγορία:	[EL] Βιοχημεία[EN] Biochemistry
Λέξεις-Κλειδιά:	Cardiomyocytes culture Cell-material interaction Chitosan-fibrinogen assemblies Polysaccharide-protein multilayers Tunable hydrophilicity/hydrophobicity
EU Grant:	LabEx REVIVE
EU Grant identifier:	ANR-10-LABX-73
Κάτοχος πνευματικών δικαιωμάτων:	© 2021 Wiley-VCH GmbH
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://onlinelibrary.wiley.com/doi/full/10.1002/mabi.202100346
Σημειώσεις:	The authors thank Dr. Georgios D. Chryssikos and Dr. Eirini Siranidi fortheir help in ATR-FTIR measurements. The authors also thank the per-sonnel of the Photon Microscopy Facility of IBPS for helpful advice andtechnical assistance during microscopy image acquisition and analysis.This work was supported by the LabEx REVIVE (ANR-10-LABX-73). Also,this work was sponsored by the Ile-de-France Region in the framework ofRespore, the Île-de-France network of Excellence in Porous Solids. MK ac-knowledges personal funding from both LabEx REVIVE and DIM Respore too.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο