Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	PnBA-b-PNIPAM-b-PDMAEA Thermo-Responsive Triblock Terpolymers and Their Quaternized Analogs as Gene and Drug Delivery Vectors
Δημιουργός/Συγγραφέας:	Skandalis, Athanasios Selianitis, Dimitrios [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios
Εκδότης:	MDPI
Ημερομηνία:	2021
Γλώσσα:	Αγγλικά
ISSN:	2073-4360
DOI:	10.3390/polym13142361
Περίληψη:	In this work, the ability of thermo-responsive poly [butyl acrylate-b-N-isopropylacrylamide-b-2-(dimethylamino) ethyl acrylate] (PnBA-b-PNIPAM-b-PDMAEA) triblock terpolymer self-assemblies, as well as of their quaternized analogs (PnBA-b-PNIPAM-b-QPDMAEA), to form polyplexes with DNA through electrostatic interactions was examined. Terpolymer/DNA polyplexes were prepared in three different amine over phosphate group ratios (N/P), and linear DNA with a 2000 base pair length was used. In aqueous solutions, the terpolymers formed aggregates of micelles with mixed PNIPAM/(Q)PDMAEA coronas and PnBA cores. The PnBA-b-PNIPAM-b-PDMAEA terpolymers' micellar aggregates were also examined as carriers for the model hydrophobic drug curcumin (CUR). The complexation ability of the terpolymer with DNA was studied by UV-Vis spectroscopy and fluorescence spectroscopy by investigating ethidium bromide quenching. Fluorescence was also used for the determination of the intrinsic fluorescence of the CUR-loaded micellar aggregates. The structural characteristics of the polyplexes and the CUR-loaded aggregates were investigated by dynamic and electrophoretic light scattering techniques. Polyplexes were found to structurally respond to changes in solution temperature and ionic strength, while the intrinsic fluorescence of encapsulated CUR was increased at temperatures above ambient.
Τίτλος πηγής δημοσίευσης:	Polymers
Τόμος/Κεφάλαιο:	13
Τεύχος:	14
Σελίδες:	2361
Θεματική Κατηγορία:	[EL] Βιοχημεία[EN] Biochemistry [EL] Χημική τεχνολογία[EN] Chemical technolgy
Λέξεις-Κλειδιά:	ABC triblock terpolymers Stimuli-responsive polymers Polyplexes Drug delivery Bioimaging
EU Grant:	Strengthening Human Resources Research Potential via Doctorate Research
EU Grant identifier:	MIS-5000432
Κάτοχος πνευματικών δικαιωμάτων:	© 2021 by the authors. Licensee MDPI, Basel, Switzerland
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Ηλεκτρονική διεύθυνση με ανοικτή πρόσβαση (link):	https://www.mdpi.com/2073-4360/13/14/2361
Σημειώσεις:	Funding: This research is co-financed by Greece and the European Union (European Social Fund-ESF) through the Operational Program “Human Resources Development, Education and Lifelong Learning” in the context of the project “Strengthening Human Resources Research Potential via Doctorate Research” (MIS-5000432), implemented by the State Scholarships Foundation (IKY).
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο