Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Physicochemical Properties and Biological Performance of Polymethacrylate Based Gene Delivery Vector Systems: Influence of Amino Functionalities
Δημιουργός/Συγγραφέας:	Haladjova, Emi Chrysostomou, Varvara Petrova, Maria Ugrinova, Iva [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios Rangelov, Stanislav
Εκδότης:	Wiley-VCH GmbH
Ημερομηνία:	2021-02
Γλώσσα:	Αγγλικά
ISSN:	1616-5187 1616-5195
DOI:	10.1002/mabi.202000352
Άλλο:	33283423
Περίληψη:	Physicochemical characteristics and biological performance of polyplexes based on two identical copolymers bearing tertiary amino or quaternary ammonium groups are evaluated and compared. Poly(2-(dimethylamino)ethyl methacrylate)-b-poly(oligo(ethylene glycol) methyl ether methacrylate) block copolymer (PDMAEMA-b-POEGMA) is synthesized by reversible addition fragmentation chain transfer polymerization. The tertiary amines of PDMAEMA are converted to quaternary ammonium groups by quaternization with methyl iodide. The two copolymers spontaneously formed well-defined polyplexes with DNA. The size, zeta potential, molar mass, aggregation number, and morphology of the polyplex particles are determined. The parent PDMAEMA-b-POEGMA exhibits larger buffering capacity, whereas the corresponding quaternized copolymer (QPDMAEMA-b-POEGMA) displays stronger binding affinity to DNA, yielding invariably larger in size and molar mass particles bearing greater number of DNA molecules per particle. Experiments revealed that QPDMAEMA-b-POEGMA is more effective in transfecting pEGFP-N1 than the parent copolymer, attributed to the larger size, molar mass, and DNA cargo, as well as to the effective cellular traffic, which dominated over the enhanced ability for endo-lysosomal escape of PDMAEMA-b-POEGMA.
Τίτλος πηγής δημοσίευσης:	Macromolecular Bioscience
Τόμος/Κεφάλαιο:	21
Τεύχος:	2
Θεματική Κατηγορία:	[EL] Χημεία (Γενικά)[EN] Chemistry (General) [EL] Βιοχημεία[EN] Biochemistry [EL] Φασματοσκοπία[EN] Spectroscopy
Λέξεις-Κλειδιά:	DNA/gene delivery Block co-polymethacrylates Cationic polyelectrolytes Polyplexes Amines Buffers Cell Death Cell Line, Tumor Genetic Vectors HEK293 Cells Humans Hydrodynamics Inhibitory Concentration 50 Methacrylates Nylons Particle Size Polyethylene Glycols Static Electricity Ultracentrifugation Chemical Phenomena Gene Transfer Techniques
EU Grant identifier:	D01-217/30.11.2018
Κάτοχος πνευματικών δικαιωμάτων:	© 2020 Wiley-VCH GmbH
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://onlinelibrary.wiley.com/doi/full/10.1002/mabi.202000352
Σημειώσεις:	Research Funding: Bulgarian Ministry of Education and Science. Grant Number: D01-217/30.11.2018
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο