Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Geriatric Frailty Is Associated With Oxidative Stress, Accumulation, and Defective Repair of DNA Double-Strand Breaks Independently of Age and Comorbidities
Δημιουργός/Συγγραφέας:	Kravvariti, Evrydiki Ntouros, Panagiotis A Vlachogiannis, Nikolaos I Pappa, Maria [EL] Σουλιώτης, Βασίλης Λ.[EN] Souliotis, Vassilis L. Sfikakis, Petros P.
Ημερομηνία:	2022
Γλώσσα:	Αγγλικά
ISSN:	1079-5006 1758-535X
DOI:	10.1093/gerona/glac214
Άλλο:	36209410
Περίληψη:	Defects in the DNA damage response and repair (DDR/R) network accumulate during the aging process. Physical frailty, a state of reduced physiological function and decreased resilience to biological stressors, is also exacerbated by aging, but its link with DDR/R aberrations beyond the effect of age and comorbidities is unclear. Fifty-three community-dwelling older adults, aged 65-102 years, who underwent frailty classification according to the Rockwood Clinical Frailty Scale (CFS), and 51 healthy adults younger than 45 years were examined in parallel. The following DDR/R parameters were determined in their peripheral blood mononuclear cells (PBMCs): (a) oxidative stress and abasic (apurinic/apyrimidinic; AP) sites, (b) endogenous DNA damage (alkaline comet assay olive tail moment [OTM] indicative of DNA single-strand breaks [SSBs] and double-strand breaks [DSBs] and γH2AX levels by immunofluorescence [DSBs only]), (c) capacity of the 2 main DNA repair mechanisms (DSB repair and nucleotide excision repair). Older individual-derived PBMCs displayed reduced-to-oxidized glutathione ratios indicative of increased levels of oxidative stress and increased AP sites, as well as increased accumulation of endogenous DNA damage (OTM and γH2AX) and defective DSB-repair capacity, compared with younger controls. These DDR/R aberrations were more pronounced in frail versus nonfrail older adults. Notably, oxidative stress, AP sites, DSBs, and DSB-repair capacity were associated with individual CFS levels after adjusting for chronological age, sex, Charlson Comorbidity Index, and polypharmacy. Geriatric frailty is independently associated with increased DNA damage formation and reduced DSB-R capacity, supporting further research into these measures as potential frailty biomarkers.
Τίτλος πηγής δημοσίευσης:	The journals of gerontology. Series A, Biological sciences and medical sciences
Τόμος/Κεφάλαιο:	78
Τεύχος:	4
Θεματική Κατηγορία:	[EL] Γενετική[EN] Genetics [EL] Γηριατρική[EN] Geriatrics [EL] Κυτταρολογία[EN] Cytology [EL] Βιοχημεία[EN] Biochemistry
Λέξεις-Κλειδιά:	DNA damage response and repair network DNA double-strand break repair Frailty Oxidative stress
EU Grant:	Special Account for Research Grants of the National and Kapodistrian University of Athens
EU Grant identifier:	No 0974
Κάτοχος πνευματικών δικαιωμάτων:	© 2022 The Author(s)
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://academic.oup.com/biomedgerontology/article/78/4/603/6754365#400202622
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο