Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Effect of dietary Spirulina (Arthrospira platensis) on the intestinal function of post-weaned piglet: An approach combining proteomics, metabolomics and histological studies
Δημιουργός/Συγγραφέας:	Martins, Cátia F Ribeiro, David M Matzapetakis, Manolis Pinho, Mário A Kuleš, Josipa Horvatić, Anita Prates, José A M Guillemin, Nicolas Eckersall, Peter David Freire, João P B de Almeida, André M Horvatić, Anita Prates, José A M
Ημερομηνία:	2022-10-30
Γλώσσα:	Αγγλικά
ISSN:	18743919
DOI:	10.1016/j.jprot.2022.104726
Άλλο:	36096433
Περίληψη:	The effect of dietary Spirulina (Arthrospira platensis) and CAZyme supplementation was assessed on the gut of weaned piglets, using an integrated NMR-metabolomics approach combined with Tandem Mass Tag labelled proteomics. Thirty weaned male piglets were assigned to one of the three following diets (n = 10): cereal and soybean meal basal diet (Control), basal diet with 10% Spirulina inclusion (SP) and SP diet supplemented with 0.01% lysozyme (SP + L). The experiment lasted 4 weeks and, upon slaughter, small intestine samples were collected for histological, metabolomic and proteomic analysis. No significant differences were found for the histology and metabolomics analysis between the three experimental groups. Lactate, glutamate, glycine and myo-inositol were the most abundant metabolites. Proteomics results showed 1502 proteins identified in the intestine tissue. A total of 23, 78, 27 differentially abundant proteins were detected respectively for the SP vs. Control, SP + L vs. Control and SP + L vs. SP comparisons. The incorporation of Spirulina and supplementation of lysozyme in the piglet's diets is associated to intestinal proteomic changes. These include increased protein synthesis and abundance of contractile apparatus proteins, related with increased nutrient availability, which has beneficial (increased glucose uptake) and detrimental (increased digesta viscosity) metabolic effects. SIGNIFICANCE: The use of conventional feedstuffs becomes increasingly prohibitive due to its environmental toll. To increase the sustainability of the livestock sector, novel feedstuffs such as microalgae need to be considered. However, its recalcitrant cell wall has antinutritional effects that can inhibit high dietary inclusion levels. The supplementation with CAZymes is a possible solution to this issue. The small intestine is a central piece in monogastric digestion and of particular importance for the weaned piglet. Studying the effect of dietary Spirulina and CAZyme supplementation on its histomorphology, metabolome and proteome allows studying relevant physiological adaptations to these diets.
Τίτλος πηγής δημοσίευσης:	Journal of proteomics
Τόμος/Κεφάλαιο:	269
Θεματική Κατηγορία:	[EL] Μικροβιολογία[EN] Microbiology [EL] Χημική Βιολογία[EN] Chemical Biology [EL] Βιοτεχνολογία[EN] Biotechnology [EL] Κτηνοτροφία[EN] Animal culture [EL] Βιοχημεία[EN] Biochemistry
Λέξεις-Κλειδιά:	Intestine Metabolomics Piglets Proteomics Spirulina
EU Grant identifier:	PTDC/CVT-NUT/5931/2014 08/SI/3399/2015 UIDB/00276/2020 UIDB/04129/2020 621394 SFRH/BD/143992/2019
Κάτοχος πνευματικών δικαιωμάτων:	© 2022 Elsevier B.V. All rights reserved.
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.sciencedirect.com/science/article/pii/S1874391922002500
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο