Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Three-Dimensional Nucleic Acid Nanostructures Based on Self-Assembled Polymer-Oligonucleotide Conjugates of Comblike and Coil-Comb Chain Architectures
Δημιουργός/Συγγραφέας:	Dimitrov, Erik Toncheva-Moncheva, Natalia Doumanov, Jordan A Mladenova, Kirilka Petrova, Svetla [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios Rangelov, Stanislav
Ημερομηνία:	2023-05-08
Γλώσσα:	Αγγλικά
ISSN:	1525-7797 1526-4602
DOI:	10.1021/acs.biomac.3c00126
Άλλο:	37014992
Περίληψη:	Spherical nucleic acids have emerged as a class of nanostructures, exhibiting a wide variety of properties, distinctly different from those of linear nucleic acids, and a plethora of applications in therapeutics and diagnostics. Herein, we report on preparation of 3D nucleic acid nanostructures, prepared by self-assembly of polymer-oligonucleotide conjugates. The latter are obtained by grafting multiple alkyne-functionalized oligonucleotide strands onto azide-modified homo-, block, and random (co)polymers of chloromethylstyrene via initiator-free click coupling chemistry to form conjugates of comblike and coil-comb chain architectures. The resulting conjugates are amphiphilic and form stable nanosized supramolecular structures in aqueous solution. The nanoconstructs are thoroughly investigated and a number of physical characteristics, in particular, molar mass, size, aggregation number, zeta potential, material density, number of oligonucleotide strands per particle, grafting density, and their relation to hallmark properties of spherical nucleic acids - biocompatibility, resistance against DNase I, cellular uptake without the need for transfection agents - are determined.
Τίτλος πηγής δημοσίευσης:	Biomacromolecules
Τόμος/Κεφάλαιο:	24
Τεύχος:	5
Σελίδες:	2213−2224
Θεματική Κατηγορία:	[EL] Φυσική και θεωρητική χημεία[EN] Physical and theoretical chemistry [EL] Νανοτεχνολογία[EN] Nanotechnology [EL] Χημική Βιολογία[EN] Chemical Biology
Λέξεις-Κλειδιά:	Polymers Oligonucleotides Click Chemistry Nucleic Acids Nanostructures
EU Grant identifier:	DN19/8-2017
Κάτοχος πνευματικών δικαιωμάτων:	© 2023 American Chemical Society
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://pubs.acs.org/doi/10.1021/acs.biomac.3c00126
Σημειώσεις:	The Supporting Information is available at https://pubs.acs.org/doi/10.1021/acs.biomac.3c00126.
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο