Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	(Hydroxypropyl)methyl Cellulose-Chitosan Film as a Matrix for Lipase Immobilization-Part ΙΙ: Structural Studies
Δημιουργός/Συγγραφέας:	Vassiliadi, Evdokia Tsirigotis-Maniecka, Marta Symons, Henry E Gobbo, Pierangelo Nallet, Frédéric [EL] Ξενάκης, Αριστοτέλης[EN] Xenakis, Aristotelis [EL] Ζουμπανιώτη, Μαρία[EN] Zoumpanioti, Maria
Ημερομηνία:	2022-09-17
Γλώσσα:	Αγγλικά
ISSN:	2310-2861
DOI:	10.3390/gels8090595
Άλλο:	36135307
Περίληψη:	The present work reports on the structural study of a film made of a hybrid blend of biopolymers used as an enzyme carrier. A cellulose derivative (HPMC) and chitosan (CS) were combined in order to formulate a film on which Mucor miehei lipase was immobilized. The film was successfully used as a biocatalyst; however, little is known about the structure of the system. Therefore, small-angle X-ray scattering, Fourier transform infrared spectroscopy (FTIR), optical microscopy, and scanning electron microscopy (SEM), as well as microindentation measurements, were used to shed light on the structure of the promising biocatalyst. Among the results, intermolecular hydrogen bonds were observed between the amide groups of the two polymers and the lipase. The presence of the enzyme does not seem to affect the mechanical properties of the matrix. The used film after 35 cycles of reaction seemed to be fatigued and had lost part of its humidity, explaining the reduction of the enzyme activity.
Τίτλος πηγής δημοσίευσης:	Gels (Basel, Switzerland)
Τόμος/Κεφάλαιο:	8
Τεύχος:	9
Θεματική Κατηγορία:	[EL] Βιοτεχνολογία[EN] Biotechnology [EL] Βιοχημεία[EN] Biochemistry [EL] Φασματοσκοπία[EN] Spectroscopy
Λέξεις-Κλειδιά:	Blended polymers Characterization Enzyme immobilization Film Hydroxypropylmethyl cellulose Lipase
EU Grant identifier:	EP/T01508X/1
Κάτοχος πνευματικών δικαιωμάτων:	© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license(https://creativecommons.org/licenses/by/ 4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.mdpi.com/2310-2861/8/9/595
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο