Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	In Vitro Evaluation of Curcumin- and Quercetin-Loaded Nanoemulsions for Intranasal Administration: Effect of Surface Charge and Viscosity
Δημιουργός/Συγγραφέας:	Vaz, Gustavo Clementino, Adryana Mitsou, Evgenia Ferrari, Elena Buttini, Francesca Sissa, Cristina [EL] Ξενάκης, Αριστοτέλης[EN] Xenakis, Aristotelis Sonvico, Fabio Dora, Cristiana Lima
Ημερομηνία:	2022-01-14
Γλώσσα:	Αγγλικά
ISSN:	1999-4923
DOI:	10.3390/pharmaceutics14010194
Άλλο:	35057089
Περίληψη:	The nose-to-brain delivery of neuroprotective natural compounds is an appealing approach for the treatment of neurodegenerative diseases. Nanoemulsions containing curcumin (CUR) and quercetin (QU) were prepared by high-pressure homogenization and characterized physicochemically and structurally. A negative (CQ_NE-), a positive (CQ_NE+), and a gel (CQ_NEgel) formulation were developed. The mean particle size of the CQ_NE- and CQ_NE+ was below 120 nm, while this increased to 240 nm for the CQ_NEgel. The formulations showed high encapsulation efficiency and protected the CUR/QU from biological/chemical degradation. Electron paramagnetic resonance spectroscopy showed that the CUR/QU were located at the interface of the oil phase in the proximity of the surfactant layer. The cytotoxicity studies showed that the formulations containing CUR/QU protected human nasal cells from the toxicity evidenced for blank NEs. No permeation across an in vitro model nasal epithelium was evidenced for CUR/QU, probably due to their poor water-solubility and instability in physiological buffers. However, the nasal cells' drug uptake showed that the total amount of CUR/QU in the cells was related to the NE characteristics (CQ_NE- > CQ_NE+ > CQ_NEgel). The method used allowed the obtainment of nanocarriers of an appropriate size for nasal administration. The treatment of the cells showed the protection of cellular viability, holding promise as an anti-inflammatory treatment able to prevent neurodegenerative diseases.
Τίτλος πηγής δημοσίευσης:	Pharmaceutics
Τόμος/Κεφάλαιο:	14
Τεύχος:	1
Θεματική Κατηγορία:	[EL] Χημική Βιολογία[EN] Chemical Biology [EL] Φασματοσκοπία[EN] Spectroscopy [EL] Νανοτεχνολογία[EN] Nanotechnology [EL] Βιοτεχνολογία[EN] Biotechnology [EL] Αναλυτική χημεία[EN] Analytical chemistry
Λέξεις-Κλειδιά:	RPMI 2650 Central nervous system Curcumin Electron paramagnetic resonance spectroscopy Nose-to-brain delivery Quercetin
EU Grant:	Programa Doutorado-Sanduíche no Exterior
EU Grant identifier:	PDSE-88881.189309/2018-01
Κάτοχος πνευματικών δικαιωμάτων:	© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.mdpi.com/1999-4923/14/1/194
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο