Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Immunomodulating Activity of Pleurotus eryngii Mushrooms Following Their In Vitro Fermentation by Human Fecal Microbiota
Δημιουργός/Συγγραφέας:	Vlassopoulou, Marigoula Paschalidis, Nikolaos Savvides, Alexandros L Saxami, Georgia Mitsou, Evdokia K Kerezoudi, Evangelia N Koutrotsios, Georgios Zervakis, Georgios I [EL] Γεωργιάδης, Παναγιώτης[EN] Georgiadis, Panagiotis Kyriacou, Adamantini [EL] Πλέτσα, Βασιλική[EN] Pletsa, Vassiliki
Ημερομηνία:	2022-03-22
Γλώσσα:	Αγγλικά
ISSN:	2309-608X
DOI:	10.3390/jof8040329
Άλλο:	35448559
Περίληψη:	Recent studies have revealed the crucial role of several edible mushrooms and fungal compounds, mainly polysaccharides, in human health and disease. The investigation of the immunomodulating effects of mushroom polysaccharides, especially β-glucans, and the link between their anticancer and immunomodulatory properties with their possible prebiotic activity on gut micro-organisms has been the subject of intense research over the last decade. We investigated the immunomodulating effects of Pleurotus eryngii mushrooms, selected due to their high β-glucan content, strong lactogenic effect, and potent geno-protective properties, following in vitro fermentation by fecal inocula from healthy elderly volunteers (>60 years old). The immunomodulating properties of the fermentation supernatants (FSs) were initially investigated in U937-derived human macrophages. Gene expression as well as pro- (TNF-α, IL-1β) and anti-inflammatory cytokines (IL-10, IL-1Rα) were assessed and correlated with the fermentation process. The presence of P. eryngii in the fermentation process led to modifications in immune response, as indicated by the altered gene expression and levels of the cytokines examined, a finding consistent for all volunteers. The FSs immunomodulating effect on the volunteers’ peripheral blood mononuclear cells (PBMCs) was verified through the use of cytometry by time of flight (CyTOF) analysis.
Τίτλος πηγής δημοσίευσης:	Journal of fungi (Basel, Switzerland)
Τόμος/Κεφάλαιο:	8
Τεύχος:	4
Θεματική Κατηγορία:	[EL] Βιοχημεία[EN] Biochemistry [EL] Ανοσολογία[EN] Immunology [EL] Φυσιολογία[EN] Physiology [EL] Χημική Βιολογία[EN] Chemical Biology [EL] Μικροβιολογία[EN] Microbiology
Λέξεις-Κλειδιά:	PBMCs Cytokines Edible mushrooms Macrophages
EU Grant:	RESEARCH-CREATE-INNOVATE
EU Grant identifier:	T1EDK-03404
Κάτοχος πνευματικών δικαιωμάτων:	© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.mdpi.com/2309-608X/8/4/329
Σημειώσεις:	Supporting information can be downloaded at https://www.mdpi.com/article/10.3390/jof8040329/s1
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο