Εξειδίκευση τύπου :	Κεφάλαιο βιβλίου
Τίτλος:	The Discovery of Peptide Macrocycle Rescuers of Pathogenic Protein Misfolding and Aggregation by Integrating SICLOPPS Technology and Ultrahigh-Throughput Screening in Bacteria
Δημιουργός/Συγγραφέας:	Delivoria, Dafni C [EL] Σκρέτας, Γιώργος[EN] Skretas, George
Επιμελητής έκδοσης:	Coppock, M.B Winton, Alexander J.
Εκδότης:	Humana Press
Ημερομηνία:	2022
Γλώσσα:	Αγγλικά
ISBN:	978-1-0716-1688-8 978-1-0716-1689-5
ISSN:	1064-3745 1940-6029
DOI:	10.1007/978-1-0716-1689-5_12
Άλλο:	34596851
Περίληψη:	The phenomenon of protein misfolding and aggregation has been widely associated with numerous human diseases, such as Alzheimer's disease, systemic amyloidosis and type 2 diabetes, the vast majority of which remain incurable. To advance early stage drug discovery against these diseases, investigation of molecular libraries with expanded diversities and ultrahigh-throughput screening methodologies that allow deeper investigation of chemical space are urgently required. Toward this, we describe how Escherichia coli can be engineered so as to enable (1) the production of expanded combinatorial libraries of short, drug-like, head-to-tail cyclic peptides and (2) their simultaneous functional screening for identifying effective inhibitors of protein misfolding and aggregation using a genetic assay that links protein folding and misfolding to cell fluorescence. In this manner, cyclic peptides with the ability to inhibit pathogenic protein misfolding and/or aggregation can be readily selected by flow cytometric cell sorting in an ultrahigh-throughput fashion. This biotechnological approach accelerates significantly the identification of hit/lead molecules with potentially therapeutic properties against devastating diseases.
Τίτλος πηγής δημοσίευσης:	eptide Macrocycles. Methods in Molecular Biology
Τόμος/Κεφάλαιο:	2371
Σελίδες:	215–246
Σειρά δημοσίευσης:	Methods in Molecular Biology
Θεματική Κατηγορία:	[EL] Βιοτεχνολογία[EN] Biotechnology [EL] Δομική Βιολογία[EN] Structural Biology [EL] Μικροβιολογία[EN] Microbiology [EL] Φαρμακευτική τεχνολογία[EN] Pharmaceutical technology [EL] Φασματοσκοπία[EN] Spectroscopy
Λέξεις-Κλειδιά:	Cyclic peptides High-throughput screening Protein aggregation Protein misfolding diseases SICLOPPS technology
Κάτοχος πνευματικών δικαιωμάτων:	© 2022. Springer Science+Business Media, LLC, part of Springer Nature.
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://link.springer.com/protocol/10.1007/978-1-0716-1689-5_12#citeas
Σημειώσεις:	Springer Protocols
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο