Εξειδίκευση τύπου :	Κεφάλαιο βιβλίου
Τίτλος:	A Bi-enzymatic Immobilized Nanobiocatalyst for the Biotransformation of Oleuropein to Hydroxytyrosol
Δημιουργός/Συγγραφέας:	Giannakopoulou, Archontoula Chatzikonstantinou, Alexandra V Tsakni, Aliki [EL] Χώχος, Χρήστος[EN] Chochos, Christos Houhoula, Dimitra Stamatis, Haralambos
Επιμελητής έκδοσης:	Stamatis, Haralambos
Εκδότης:	Humana Press Inc.
Ημερομηνία:	2022
Γλώσσα:	Αγγλικά
ISBN:	978-1-0716-2268-1 978-1-0716-2269-8
ISSN:	1064-3745 1940-6029
DOI:	10.1007/978-1-0716-2269-8_17
Άλλο:	35687242
Περίληψη:	Multi-enzymatic assemblies offer the opportunity of bringing in proximity several enzymes that are enabled to work together for the catalysis of multi-step reactions. Especially, the development of robust nanobiocatalytic systems comprising of several enzymes has gained considerable attention over the last few years for the catalysis of complex reactions and the production of high added-value products. In the present chapter, we describe the methodology for the development of a bi-enzymatic nanobiocatalyst consisting of the enzymes β-glucosidase from Thermotoga maritima and lipase A from Candida antarctica (CalA) co-immobilized on chitosan-coated magnetic nanoparticles. This nanobiocatalyst can be efficiently applied for the biotransformation of oleuropein to hydroxytyrosol, a reaction of increased biotechnological interest. Several techniques, as well as methodologies that are required for the characterization of the structure and the activity of such systems are also comprehensively described.
Τίτλος πηγής δημοσίευσης:	Multienzymatic Assemblies. Methods in Molecular Biology
Τόμος/Κεφάλαιο:	2487
Σελίδες:	279 - 296
Σειρά δημοσίευσης:	Methods in Molecular Biology (MIMB)
Θεματική Κατηγορία:	[EL] Νανοτεχνολογία[EN] Nanotechnology [EL] Βιοτεχνολογία[EN] Biotechnology
Λέξεις-Κλειδιά:	Biotransformation Chitosan-coated Hydroxytyrosol Lipase Magnetic nanoparticles Multi-enzymatic assemblies Nanobiocatalyst Oleuropein β-glucosidase
EU Grant:	AntiMicrOxi-Pac
EU Grant identifier:	Τ6ΥΒΠ-00232
Κάτοχος πνευματικών δικαιωμάτων:	© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://link.springer.com/protocol/10.1007/978-1-0716-2269-8_17
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο