Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	The nanostructure of polyelectrolyte complexes of QPDMAEMA-b-POEGMA copolymers and oppositely charged polyelectrolytes, and their stability in the presence of serum albumin
Δημιουργός/Συγγραφέας:	Rappoport, Sapir Chrysostomou, Varvara [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios Talmon, Yeshayahu
Ημερομηνία:	2023
Γλώσσα:	Αγγλικά
ISSN:	1744-683X 1744-6848
DOI:	10.1039/D3SM00467H
Περίληψη:	Quaternized poly(2-(dimethylamino) ethyl methacrylate)-b-poly(oligo(ethyleneglycol) methyl ether methacrylate) (QPDMAEMA-b-POEGMA) is a copolymer of a positively charged block and a non-ionic hydrophilic block. The positively charged block, QPDMAEMA, electrostatically interacts with oppositely charged polymers, e.g., poly(acrylic acid) (PAA) and DNA, to form a complex. This complex is stable in aqueous solution due to the hydrophilic block, POEGMA, which provides colloidal stability and biocompatibility. Polyplexes can be used as non-viral vectors in gene therapy. Polyplexes are essential for delivering genetic materials into cells because they protect the genetic material from degradation before reaching the target cells, thus increasing the transfection efficiency. However, currently used polyplexes show a low transfection efficiency in vivo, probably because the polyplexes are exposed to blood proteins, such as serum albumin, which cause their dissociation. The main goal of this research is the morphology characterization of QPDMAEMA-b-POEGMA complexes with the sodium salt of polyacrylic acid (NaPAA), and with DNA by cryogenic transmission electron microscopy (cryo-TEM) and small-angle X-ray scattering (SAXS). These methods give qualitative and quantitative data about the morphology of the complexes. The morphology of the complexes was examined at different charge ratios (CRs). Complexes with NaPAA form core-corona spherical micelles and vesicular structures, whereas complexes with DNA form lamellar and hexagonal structures. The QPDMAEMA-b-POEGMA and DNA complexes were also examined after exposing them to bovine serum albumin (BSA). We found that BSA does not affect the complexes for seven days. This morphology characterization is essential for better design and formulation of vectors for gene therapy and polyelectrolyte complexes for biomedical applications.
Τίτλος πηγής δημοσίευσης:	Soft Matter
Τόμος/Κεφάλαιο:	19
Τεύχος:	20
Θεματική Κατηγορία:	[EL] Νανοτεχνολογία[EN] Nanotechnology [EL] Βιοχημεία[EN] Biochemistry [EL] Φυσική και θεωρητική χημεία[EN] Physical and theoretical chemistry
Λέξεις-Κλειδιά:	Gene delivery Polyelectrolyte complexes Polyelectrolytes Copolymers
EU Grant identifier:	# 2302/20 (Y. T.)
Κάτοχος πνευματικών δικαιωμάτων:	© 2023 The Royal Society of Chemistry
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1039/D3SM00467H
Σημειώσεις:	supplementary information at: https://pubs.rsc.org/en/content/articlelanding/2023/sm/d3sm00467h
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο