Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Preparation of trypsin-based nanoparticles, colloidal properties and ability to bind bioactive compounds
Δημιουργός/Συγγραφέας:	[EL] Παπαγιαννόπουλος, Αριστείδης[EN] Papagiannopoulos, Aristeidis Selianitis, Dimitrios Chroni, Angeliki Allwang, Johannes Li, Yanan Papadakis, Christine M.
Εκδότης:	Elsevier
Ημερομηνία:	2022
Γλώσσα:	Αγγλικά
DOI:	10.1016/j.ijbiomac.2022.03.131
Περίληψη:	Nanoparticles (NPs) based on the proteolytic enzyme trypsin (TRY) were prepared by a biocompatible methodology. TRY co-assembled with the anionic polysaccharide chondroitin sulfate (CS) in complexes with well-defined distributions of radii in the range of 100–200 nm by electrostatic complexation at acidic conditions. At pH 7 the complexes were unstable and lost their monomodal size distribution which is potentially related to TRY's weak positive net surface charge and a large negative charge patch that forms at neutral pH. Thermal treatment at conditions which were not expected to interfere with TRY's proteolytic activity was used to stabilize the complexes into NPs that resisted disintegration at pH 7 taking advantage of the ability of the TRY globules to thermally aggregate. The secondary conformation of TRY within the NPs was found fairly unperturbed even after thermal treatment which is crucial for its physiological function. The CS-TRY NPs could bind and encapsulate the bioactive substances curcumin (CUR) and β-carotene (β-C) owing to TRY's hydrophobic domains. The CS-TRY NPs may be considered as a platform for the immobilized active enzyme and multifunctional NPs for hydrophobic bioactive compounds.
Τίτλος πηγής δημοσίευσης:	International Journal of Biological Macromolecules
Τόμος/Κεφάλαιο:	208
Σελίδες:	678-687
Θεματική Κατηγορία:	[EL] Φυσική και θεωρητική χημεία[EN] Physical and theoretical chemistry [EL] Νανοτεχνολογία[EN] Nanotechnology [EL] Χημική Βιολογία[EN] Chemical Biology
Λέξεις-Κλειδιά:	Polysaccharide-protein nanoparticles Complexation
Κάτοχος πνευματικών δικαιωμάτων:	2022 Elsevier B.V. All rights reserved.
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.sciencedirect.com/science/article/pii/S0141813022006018?via%3Dihub
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο