Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	ERβ1 Sensitizes and ERβ2 Desensitizes ERα-Positive Breast Cancer Cells to the Inhibitory Effects of Tamoxifen, Fulvestrant and Their Combination with All-Trans Retinoic Acid
Δημιουργός/Συγγραφέας:	Meligova, Aggeliki K. Siakouli, Dimitra Stasinopoulou, Sotiria Xenopoulou, Despoina S Zoumpouli, Maria [EL] Γάνου, Βασιλική[EN] Ganou, Vassiliki Gkotsi, Eleni-Fani [EL] Χατζηιωάννου, Αριστοτέλης[EN] Chatziioannou, Aristotelis [EL] Παπαδόδημα, Όλγα[EN] Papadodima, Olga Pilalis, Eleftherios [EL] Αλέξης, Μιχαήλ Ν.[EN] Alexis, Michael N. [EL] Μήτσιου, Δήμητρα[EN] Mitsiou, Dimitra J.
Ημερομηνία:	2023-02-13
Γλώσσα:	Αγγλικά
ISSN:	1422-0067
DOI:	10.3390/ijms24043747
Άλλο:	36835157
Περίληψη:	Adjuvant endocrine therapy (AET) is the treatment of choice for early-stage estrogen receptor alpha (ERα)-positive breast cancer (BC). However, almost 40% of tamoxifen-treated cases display no response or a partial response to AET, thus increasing the need for new treatment options and strong predictors of the therapeutic response of patients at high risk of relapse. In addition to ERα, BC research has focused on ERβ1 and ERβ2 (isoforms of ERβ), the second ER isotype. At present, the impact of ERβ isoforms on ERα-positive BC prognosis and treatment remains elusive. In the present study, we established clones of MCF7 cells constitutively expressing human ERβ1 or ERβ2 and investigated their role in the response of MCF7 cells to antiestrogens [4-hydroxytamoxifen (OHΤ) and fulvestrant (ICI182,780)] and retinoids [all-trans retinoic acid (ATRA)]. We show that, compared to MCF7 cells, MCF7-ERβ1 and MCF7-ERβ2 cells were sensitized and desensitized, respectively, to the antiproliferative effect of the antiestrogens, ATRA and their combination and to the cytocidal effect of the combination of OHT and ATRA. Analysis of the global transcriptional changes upon OHT-ATRA combinatorial treatment revealed uniquely regulated genes associated with anticancer effects in MCF7-ERβ1 cells and cancer-promoting effects in MCF7-ERβ2 cells. Our data are favorable to ERβ1 being a marker of responsiveness and ERβ2 being a marker of resistance of MCF7 cells to antiestrogens alone and in combination with ATRA.
Τίτλος πηγής δημοσίευσης:	International journal of molecular sciences
Τόμος/Κεφάλαιο:	24
Τεύχος:	4
Θεματική Κατηγορία:	[EL] Βιοχημεία[EN] Biochemistry [EL] Νεοπλάσματα. Όγκοι. Ογκολογία (περ. Καρκίνος, κακινογόνες ουσίες)[EN] Neoplasms. Tumors. Oncology (Incl.cancer, carcinogens) [EL] Χημική Βιολογία[EN] Chemical Biology [EL] Βιοπληροφορική[EN] Bioinformatics [EL] Κυτταρολογία[EN] Cytology
Λέξεις-Κλειδιά:	ATRA ERβ1 ERβ2 ICI182,780 Breast cancer Cell death Cell Proliferation Gene expression Prognostic markers Tamoxifen Female Humans Antineoplastic Combined Chemotherapy Protocols Estrogen Antagonists Estrogen Receptor alpha Estrogen Receptor Modulators Fulvestrant Neoplasm Recurrence, Local Protein Isoforms Tretinoin Breast Neoplasms Drug Resistance, Neoplasm Estrogen Receptor beta
EU Grant:	Validation—Reinforcement of the Prognostic Significance of Estrogen Receptor beta in Early Breast Cancer
EU Grant identifier:	MIS 5050141
Κάτοχος πνευματικών δικαιωμάτων:	© 2023 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.mdpi.com/1422-0067/24/4/3747
Έχει σχέση με:	https://www.ebi.ac.uk/biostudies/arrayexpress/studies/E-MTAB-12549?query=E-MTAB-12549
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο