Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	The T2 structure of polycrystalline cubic human insulin
Δημιουργός/Συγγραφέας:	Triandafillidis, Dimitris P Karavassili, Fotini Spiliopoulou, Maria Valmas, Alexandros Athanasiadou, Maria Nikolaras, George Fili, Stavroula Kontou, Paraskevi Bowler, Matthew W [EL] Χασάπης, Χρήστος[EN] Chasapis, Christos Von Dreele, Robert B Fitch, Andrew N Margiolaki, Irene
Ημερομηνία:	2023-05-01
Γλώσσα:	Αγγλικά
ISSN:	2059-7983
DOI:	10.1107/S2059798323001328
Άλλο:	37039669
Περίληψη:	The polymorphism of human insulin upon pH variation was characterized via X-ray powder diffraction, employing a crystallization protocol previously established for co-crystallization with phenolic derivatives. Two distinct rhombohedral (R3) polymorphs and one cubic (I213) polymorph were identified with increasing pH, corresponding to the T6, T3R3f and T2 conformations of insulin, respectively. The structure of the cubic T2 polymorph was determined via multi-profile stereochemically restrained Rietveld refinement at 2.7 Å resolution. This constitutes the first cubic insulin structure to be determined from crystals grown in the presence of zinc ions, although no zinc binding was observed. The differences of the polycrystalline variant from other cubic insulin structures, as well as the nature of the pH-driven phase transitions, are discussed in detail.
Τίτλος πηγής δημοσίευσης:	Acta crystallographica. Section D, Structural biology
Τόμος/Κεφάλαιο:	79
Τεύχος:	Pt 5
Θεματική Κατηγορία:	[EL] Κρυσταλλογραφία[EN] Crystallography [EL] Βιοχημεία[EN] Biochemistry [EL] Δομική Βιολογία[EN] Structural Biology
Λέξεις-Κλειδιά:	Human insulin Cubic polymorph X-ray powder diffraction Synchrotron radiation
EU Grant:	First Call for HFRI Research Projects to support Faculty members and Researchers and the procurement of high-cost research equipment grant
EU Grant identifier:	3051
Όροι και προϋποθέσεις δικαιωμάτων:	published under CCBY Licence
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://scripts.iucr.org/cgi-bin/paper?S2059798323001328
Έχει σχέση με:	https://www.wwpdb.org/pdb?id=pdb_00007qac https://journals.iucr.org/d/issues/2023/05/00/tz5109/tz5109sup1.pdf
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο