Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Chronological Age and DNA Damage Accumulation in Blood Mononuclear Cells: A Linear Association in Healthy Humans after 50 Years of Age
Δημιουργός/Συγγραφέας:	Vlachogiannis, Nikolaos I Ntouros, Panagiotis A Pappa, Maria Kravvariti, Evrydiki Kostaki, Evangelia Georgia Fragoulis, E. G. Papanikolaou, Christina Mavroeidi, Dimitra Bournia, Vasiliki-Kalliopi Panopoulos, Stylianos Laskari, Katerina Arida, Aikaterini Gorgoulis, Vassilis G Tektonidou, Maria G Paraskevis, Dimitrios Sfikakis, Petros P. [EL] Σουλιώτης, Βασίλης Λ.[EN] Souliotis, Vassilis L.
Ημερομηνία:	2023-04-12
Γλώσσα:	Αγγλικά
ISSN:	1422-0067
DOI:	10.3390/ijms24087148
Άλλο:	37108309
Περίληψη:	Aging is characterized by the progressive deregulation of homeostatic mechanisms causing the accumulation of macromolecular damage, including DNA damage, progressive decline in organ function and chronic diseases. Since several features of the aging phenotype are closely related to defects in the DNA damage response (DDR) network, we have herein investigated the relationship between chronological age and DDR signals in peripheral blood mononuclear cells (PBMCs) from healthy individuals. DDR-associated parameters, including endogenous DNA damage (single-strand breaks and double-strand breaks (DSBs) measured by the alkaline comet assay (Olive Tail Moment (OTM); DSBs-only by γH2AX immunofluorescence staining), DSBs repair capacity, oxidative stress, and apurinic/apyrimidinic sites were evaluated in PBMCs of 243 individuals aged 18-75 years, free of any major comorbidity. While OTM values showed marginal correlation with age until 50 years (rs = 0.41, p = 0.11), a linear relationship was observed after 50 years (r = 0.95, p < 0.001). Moreover, individuals older than 50 years showed increased endogenous DSBs levels (γH2Ax), higher oxidative stress, augmented apurinic/apyrimidinic sites and decreased DSBs repair capacity than those with age lower than 50 years (all p < 0.001). Results were reproduced when we examined men and women separately. Prospective studies confirming the value of DNA damage accumulation as a biomarker of aging, as well as the presence of a relevant agethreshold, are warranted.
Τίτλος πηγής δημοσίευσης:	International journal of molecular sciences
Τόμος/Κεφάλαιο:	24
Τεύχος:	8
Θεματική Κατηγορία:	[EL] Βιοχημεία[EN] Biochemistry [EL] Γενετική[EN] Genetics [EL] Κυτταρολογία[EN] Cytology
Λέξεις-Κλειδιά:	Comet assay DNA damage response apurinic/apyrimidinic sites chronological age double-strand breaks repair capacity endogenous DNA damage oxidative stress Male Humans Female Middle Aged Prospective Studies DNA Damage Aging DNA Repair Leukocytes, Mononuclear DNA Breaks, Double-Stranded
EU Grant:	ELKE-NKUA
EU Grant identifier:	#0974
Κάτοχος πνευματικών δικαιωμάτων:	© 2023 by the authors
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.mdpi.com/1422-0067/24/8/7148
Ηλεκτρονική διεύθυνση περιοδικού (link) :	https://www.mdpi.com/
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο