Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Genome-Wide Analysis of lncRNA-mRNA Co-Expression Networks in CD133+/CD44+ Stem-like PDAC Cells
Δημιουργός/Συγγραφέας:	Eptaminitaki, Giasemi C Zaravinos, Apostolos [EL] Στέλλας, Δημήτρης[EN] Stellas, Dimitris Panagopoulou, Maria Karaliota, Sevasti Baltsavia, Ismini Iliopoulos, Ioannis Chatzaki, Ekaterini Iliopoulos, Dimitrios Baritaki, Stavroula
Ημερομηνία:	2023-02-07
Γλώσσα:	Αγγλικά
ISSN:	2072-6694
DOI:	10.3390/cancers15041053
Άλλο:	36831395
Περίληψη:	Pancreatic ductal adenocarcinoma (PDAC), the second most prevalent gastrointestinal malignancy and the most common type of pancreatic cancer is linked with poor prognosis and, eventually, with high mortality rates. Early detection is seldom, while tumor heterogeneity and microarchitectural alterations benefit PDAC resistance to conventional therapeutics. Although emerging evidence suggest the core role of cancer stem cells (CSCs) in PDAC aggressiveness, unique stem signatures are poorly available, thus limiting the efforts of anti-CSC-targeted therapy. Herein, we report the findings of the first genome-wide analyses of mRNA/lncRNA transcriptome profiling and co-expression networks in PDAC cell line-derived CD133+/CD44+ cells, which were shown to bear a CSC-like phenotype in vitro and in vivo. Compared to CD133-/CD44- cells, the CD133+/CD44+ population demonstrated significant expression differences in both transcript pools. Using emerging bioinformatic tools, we performed lncRNA target coding gene prediction analysis, which revealed significant Gene Ontology (GO), pathway, and network enrichments in many dyregulated lncRNA nearby (cis or trans) mRNAs, with reported involvement in the regulation of CSC phenotype and functions. In this context, the construction of lncRNA/mRNA networks by ingenuity platforms identified the lncRNAs ATF2, CHEK1, DCAF8, and PAX8 to interact with "hub" SC-associated mRNAs. In addition, the expressions of the above lncRNAs retrieved by TCGA-normalized RNAseq gene expression data of PAAD were significantly correlated with clinicopathological features of PDAC, including tumor grade and stage, nodal metastasis, and overall survival. Overall, our findings shed light on the identification of CSC-specific lncRNA signatures with potential prognostic and therapeutic significance in PDAC.
Τίτλος πηγής δημοσίευσης:	Cancers
Τόμος/Κεφάλαιο:	15
Τεύχος:	4
Θεματική Κατηγορία:	[EL] Νεοπλάσματα. Όγκοι. Ογκολογία (περ. Καρκίνος, κακινογόνες ουσίες)[EN] Neoplasms. Tumors. Oncology (Incl.cancer, carcinogens) [EL] Βιοχημεία[EN] Biochemistry [EL] Βιοπληροφορική[EN] Bioinformatics [EL] Γενετική[EN] Genetics [EL] Κυτταρολογία[EN] Cytology
Λέξεις-Κλειδιά:	Cancer biomarkers Cancer stem cells (CSCs) Long non-coding RNAs (lncRNAs) Pancreatic ductal adenocarcinoma (PDAC)
EU Grant:	1st Call for H.F.R.I. Research Projects to support Faculty Members & Researchers and Procure High-Value Research Equipment
EU Grant identifier:	H.F.R.I-FM17-3099, PI: S.B.
Κάτοχος πνευματικών δικαιωμάτων:	: © 2023 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.mdpi.com/2072-6694/15/4/1053
Έχει σχέση με:	https://www.ncbi.nlm.nih.gov/medgen?linkname=pubmed_medgen&from_uid=36831395
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο