Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	A fluorophore-conjugated reagent enabling rapid detection, isolation and live tracking of senescent cells
Δημιουργός/Συγγραφέας:	Magkouta, Sophia Veroutis, Dimitris Pousias, Athanasios Papaspyropoulos, Angelos Pippa, Natassa Lougiakis, Nikolaos Kambas, Konstantinos Lagopati, Nefeli Polyzou, Aikaterini Georgiou, Maria Chountoulesi, Maria [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios Foutadakis, Spyros Pouli, Nicole Marakos, Panagiotis Kotsinas, Athanassios Verginis, Panayotis Valakos, Dimitrios Mizi, Athanasia Papantonis, Argyris Vatsellas, Giannis Galanos, Panagiotis Bartek, Jiri Petty, Russell Serrano, Manuel Thanos, Dimitris Roussos, Charis Demaria, Marco Evangelou, Konstantinos Gorgoulis, Vassilis G
Εκδότης:	Molecular Cell
Ημερομηνία:	2023-10-05
Γλώσσα:	Αγγλικά
DOI:	10.1016/j.molcel.2023.09.006
Άλλο:	37802028
Περίληψη:	Cellular senescence is a stress-response mechanism implicated in various physiological processes, diseases, and aging. Current detection approaches have partially addressed the issue of senescent cell identification in clinical specimens. Effective methodologies enabling precise isolation or live tracking of senescent cells are still lacking. In-depth analysis of truly senescent cells is, therefore, an extremely challenging task. We report (1) the synthesis and validation of a fluorophore-conjugated, Sudan Black-B analog (GLF16), suitable for in vivo and in vitro analysis of senescence by fluorescence microscopy and flow cytometry and (2) the development and application of a GLF16-carrying micelle vector facilitating GLF16 uptake by living senescent cells in vivo and in vitro. The compound and the applied methodology render isolation of senescent cells an easy, rapid, and precise process. Straightforward nanocarrier-mediated GLF16 delivery in live senescent cells comprises a unique tool for characterization of senescence at an unprecedented depth.
Τίτλος πηγής δημοσίευσης:	Molecular cell
Τόμος/Κεφάλαιο:	83
Τεύχος:	19
Θεματική Κατηγορία:	[EL] Ιατρική[EN] Medicine [EL] Χημική Βιολογία[EN] Chemical Biology
Λέξεις-Κλειδιά:	GLF-16 flow cytometry fluorescence microscopy live tracking micelle optimized SBB analogs senescence
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1016/j.molcel.2023.09.006
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο