Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Thermally stabilized chondroitin sulfate-hemoglobin nanoparticles and their interaction with bioactive compounds
Δημιουργός/Συγγραφέας:	[EL] Παπαγιαννόπουλος, Αριστείδης[EN] Papagiannopoulos, Aristeidis Sklapani, Aggeliki Spiliopoulos, Nikolaos
Ημερομηνία:	2023-10-29
Γλώσσα:	Αγγλικά
ISSN:	03014622
DOI:	10.1016/j.bpc.2023.107127
Άλλο:	37952498
Περίληψη:	The preparation of nanoparticles (NPs) based on hemoglobin (Hb) with a fully biocompatible methodology is presented. The spontaneous formation of electrostatic complexes of Hb with chondroitin sulfate (CS) at pH 4 in the polysaccharide/protein mass ratio regime where charge neutrality is met leads to spherical nanostructures with monomodal hydrodynamic radii distribution in the range of 50-100 nm. The integrity of the electrostatic complexes is disturbed at pH 7 as the net electric charge of Hb is very low. Treating the NPs at mildly elevated temperature stabilizes them against the pH increase taking advantage of Hb's ability of unfolding and self-associating upon thermal treatment. The NPs surface charge is pH-tunable and changes from positive to strongly negative upon pH increase to 7 proving the presence of negative surface patches of Hb and CS segments in their exterior. The α-helix content of Hb does not change significantly by thermal treatment. The NPs are found to bind the bioactive compounds curcumin and β-carotene and are stable in solutions with high salt content. This investigation introduces a straightforward method to formulate Hb in NPs with possibilities in the nanodelivery of nutrients and drugs.
Τίτλος πηγής δημοσίευσης:	Biophysical chemistry
Τόμος/Κεφάλαιο:	304
Θεματική Κατηγορία:	[EL] Φυσική και θεωρητική χημεία[EN] Physical and theoretical chemistry [EL] Φασματοσκοπία[EN] Spectroscopy [EL] Νανοτεχνολογία[EN] Nanotechnology
Λέξεις-Κλειδιά:	Electrostatic complexation Light scattering Polysaccharides Proteins Spectroscopy Chondroitin Sulfates Hemoglobins Nanoparticles Nanostructures Curcumin
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1016/j.bpc.2023.107127
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο