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Εξειδίκευση τύπου : Άρθρο σε επιστημονικό περιοδικό
Τίτλος: Cryo-EM structure of a plant photosystem II supercomplex with light-harvesting protein Lhcb8 and α-tocopherol
Δημιουργός/Συγγραφέας: Opatíková, Monika
Semchonok, Dmitry A
Kopečný, David
Ilík, Petr
Pospíšil, Pavel
Ilíková, Iva
Roudnický, Pavel
Zeljković, Sanja Ćavar
Tarkowski, Petr
Kyrilis, Fotis L
Hamdi, Farzad
[EL] Καστρίτης, Παναγιώτης[EN] Kastritis, Panagiotissemantics logo
Kouřil, Roman
Ημερομηνία: 2023-08
Γλώσσα: Αγγλικά
ISSN: 2055-0278
DOI: 10.1038/s41477-023-01483-0
Άλλο: 37550369
Περίληψη: The heart of oxygenic photosynthesis is the water-splitting photosystem II (PSII), which forms supercomplexes with a variable amount of peripheral trimeric light-harvesting complexes (LHCII). Our knowledge of the structure of green plant PSII supercomplex is based on findings obtained from several representatives of green algae and flowering plants; however, data from a non-flowering plant are currently missing. Here we report a cryo-electron microscopy structure of PSII supercomplex from spruce, a representative of non-flowering land plants, at 2.8 Å resolution. Compared with flowering plants, PSII supercomplex in spruce contains an additional Ycf12 subunit, Lhcb4 protein is replaced by Lhcb8, and trimeric LHCII is present as a homotrimer of Lhcb1. Unexpectedly, we have found α-tocopherol (α-Toc)/α-tocopherolquinone (α-TQ) at the boundary between the LHCII trimer and the inner antenna CP43. The molecule of α-Toc/α-TQ is located close to chlorophyll a614 of one of the Lhcb1 proteins and its chromanol/quinone head is exposed to the thylakoid lumen. The position of α-Toc in PSII supercomplex makes it an ideal candidate for the sensor of excessive light, as α-Toc can be oxidized to α-TQ by high-light-induced singlet oxygen at low lumenal pH. The molecule of α-TQ appears to shift slightly into the PSII supercomplex, which could trigger important structure-functional modifications in PSII supercomplex. Inspection of the previously reported cryo-electron microscopy maps of PSII supercomplexes indicates that α-Toc/α-TQ can be present at the same site also in PSII supercomplexes from flowering plants, but its identification in the previous studies has been hindered by insufficient resolution.
Τίτλος πηγής δημοσίευσης: Nature plants
Τόμος/Κεφάλαιο: 9
Τεύχος: 8
Σελίδες: 1359–1369
Θεματική Κατηγορία: [EL] Φυσιολογία φυτών[EN] Plant physiologysemantics logo
[EL] Εφαρμοσμένη οπτική. Φωτονική[EN] Applied optics. Photonicssemantics logo
[EL] Βιοχημεία[EN] Biochemistrysemantics logo
Λέξεις-Κλειδιά: Cryoelectron Microscopy
Thylakoids
Photosynthesis
Plants
Photosystem II Protein Complex
alpha-Tocopherol
EU Grant: Plants as a tool for sustainable global development
BMBF, ZIK programme
hot4cryo
Saxony-Anhalt
Sustainable systems and technologies, improving crop production for higher quality of production of food, feed, and raw materials, under conditions of changing climate
UP CIISB
EU Grant identifier: 21-05497S
CZ.02.1.01/0.0/0.0/16_019/0000827
03Z22HN23
03Z22HI2
03COV04
101086665
ZS/2016/04/78115
391498659
RTG 2467
RO0423
LM2023042
CZ.02.1.01/0.0/0.0/18_046/0015974
Κάτοχος πνευματικών δικαιωμάτων: © 2023, The Author(s), under exclusive licence to Springer Nature Limited.
Ηλεκτρονική διεύθυνση στον εκδότη (link): https://doi.org/10.1038/s41477-023-01483-0
Εμφανίζεται στις συλλογές:Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο

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