Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Genotoxic effects of occupational exposure to glass fibres - A human biomonitoring study
Δημιουργός/Συγγραφέας:	Ceppi, Marcello Smolkova, Bozena Staruchova, Marta Kazimirova, Alena Barancokova, Magdalena Volkovova, Katarina Collins, Andrew Kocan, Anton Dzupinkova, Zuzana Horska, Alexandra Buocikova, Verona Tulinska, Jana Liskova, Aurelia Mikusova, Miroslava Lehotska Krivosikova, Zora Wsolova, Ladislava Kuba, Daniel Rundén-Pran, Elise El Yamani, Naouale Longhin, Eleonora Martha Halašová, Erika [EL] Κυρτόπουλος, Σωτήριος Α.[EN] Kyrtopoulos, Soterios A. Bonassi, Stefano Dusinska, Maria
Ημερομηνία:	2023-01
Γλώσσα:	Αγγλικά
ISSN:	13835718
DOI:	10.1016/j.mrgentox.2022.503572
Άλλο:	36669817
Περίληψη:	As part of a large human biomonitoring study, we conducted occupational monitoring in a glass fibre factory in Slovakia. Shopfloor workers (n = 80), with a matched group of administrators in the same factory (n = 36), were monitored for exposure to glass fibres and to polycyclic aromatic hydrocarbons (PAHs). The impact of occupational exposure on chromosomal aberrations, DNA damage and DNA repair, immunomodulatory markers, and the role of nutritional and lifestyle factors, as well as the effect of polymorphisms in metabolic and DNA repair genes on genetic stability, were investigated. The (enzyme-modified) comet assay was employed to measure DNA strand breaks (SBs) and apurinic sites, oxidised and alkylated bases. Antioxidant status was estimated by resistance to H2O2-induced DNA damage. Base excision repair capacity was measured with an in vitro assay (based on the comet assay). Exposure of workers to fibres was low, but still was associated with higher levels of SBs, and SBs plus oxidised bases, and higher sensitivity to H2O2. Multivariate analysis showed that exposure increased the risk of high levels of SBs by 20%. DNA damage was influenced by antioxidant enzymes catalase and glutathione S-transferase (measured in blood). DNA repair capacity was inversely correlated with DNA damage and positively with antioxidant status. An inverse correlation was found between DNA base oxidation and the percentage of eosinophils (involved in the inflammatory response) in peripheral blood of both exposed and reference groups. Genotypes of XRCC1 variants rs3213245 and rs25487 significantly decreased the risk of high levels of base oxidation, to 0.50 (p = 0.001) and 0.59 (p = 0.001), respectively. Increases in DNA damage owing to glass fibre exposure were significant but modest, and no increases were seen in chromosome aberrations or micronuclei. However, it is of concern that even low levels of exposure to these fibres can cause significant genetic damage.
Τίτλος πηγής δημοσίευσης:	Mutation research. Genetic toxicology and environmental mutagenesis
Τόμος/Κεφάλαιο:	885
Θεματική Κατηγορία:	[EL] Βιοχημεία[EN] Biochemistry [EL] Γενετική[EN] Genetics [EL] Μοριακή Βιολογία[EN] Molecular Biology [EL] Περιβαλλοντική υγεία[EN] Environmental health
Λέξεις-Κλειδιά:	Biomarkers DNA instability Genetic polymorphism Glass fibre Human biomonitoring Immunomodulatory markers Occupational exposure
EU Grant:	FIBRETOX hCOMET VISION LISPER PARC
EU Grant identifier:	QLK4–1999-01629 CA15132 857381 IMTS:313011V446 101057014
Κάτοχος πνευματικών δικαιωμάτων:	© 2022 The Authors. Published by Elsevier B.V.
Όροι και προϋποθέσεις δικαιωμάτων:	This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1016/j.mrgentox.2022.503572
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο