Εξειδίκευση τύπου :	Κεφάλαιο βιβλίου
Τίτλος:	In-Plate and In-Gel Assays for the Assessment of Proteasome Activity in  Caenorhabditis elegans
Δημιουργός/Συγγραφέας:	Panagiotidou, Eleni Gioran, Anna [EL] Χονδρογιάννη, Νίκη[EN] Chondrogianni, Niki
Ημερομηνία:	2023
Γλώσσα:	Αγγλικά
ISBN:	978-1-0716-2858-4 978-1-0716-2859-1
ISSN:	1064-3745 1940-6029
DOI:	10.1007/978-1-0716-2859-1_16
Άλλο:	36446978
Περίληψη:	This chapter describes two methods for the study of proteasome function in Caenorhabditis elegans (C. elegans). The first method, referred to as "in-plate activities," provides a quantitative measurement of proteasome activities in C. elegans lysates by means of a kinetic reaction in a 96-well plate. The second one, referred to as "in-gel activities," involves the separation of C. elegans protein lysates in a native polyacrylamide gel and the assessment of the activity of each proteasome form. Downstream immunoblotting also allows the semi-quantitative assessment of proteasome assembly. This chapter outlines two detailed protocols along with helpful schematics and representative results that will facilitate researchers to replicate both protocols accurately and reproducibly.
Τίτλος πηγής δημοσίευσης:	Methods in molecular biology (Clifton, N.J.)
Τόμος/Κεφάλαιο:	2602
Σελίδες:	217–228
Σειρά δημοσίευσης:	Methods in Molecular Biology
Θεματική Κατηγορία:	[EL] Χημική Βιολογία[EN] Chemical Biology [EL] Βιοχημεία[EN] Biochemistry
Λέξεις-Κλειδιά:	C. elegans native-PAGE proteasome proteasome activity proteasome assembly
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://link.springer.com/protocol/10.1007/978-1-0716-2859-1_16
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο