Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Biomimetic CO2 Capture Unlocked through Enzyme Mining: Discovery of a Highly Thermo- and Alkali-Stable Carbonic Anhydrase
Δημιουργός/Συγγραφέας:	Rigkos, Konstantinos Filis, Georgios Antonopoulou, Io de Oliveira Maciel, Ayanne Saridis, Pavlos Zarafeta, Dimitra [EL] Σκρέτας, Γιώργος[EN] Skretas, George
Ημερομηνία:	2024-09-23
Γλώσσα:	Αγγλικά
ISSN:	0013-936X 1520-5851
DOI:	10.1021/acs.est.4c04291
Άλλο:	39311668
Περίληψη:	Taking immediate action to combat the urgent threat of CO2-driven global warming is crucial for ensuring a habitable planet. Decarbonizing the industrial sector requires implementing sustainable carbon-capture technologies, such as biomimetic hot potassium carbonate capture (BioHPC). BioHPC is superior to traditional amine-based strategies due to its eco-friendly nature. This innovative technology relies on robust carbonic anhydrases (CAs), enzymes that accelerate CO2 hydration and endure harsh industrial conditions like high temperature and alkalinity. Thus, the discovery of highly stable CAs is crucial for the BioHPC technology advancement. Through high-throughput bioinformatics analysis, we identified a highly thermo- and alkali-stable CA, termed CA-KR1, originating from a metagenomic sample collected at a hot spring in Kirishima, Japan. CA-KR1 demonstrates remarkable stability at high temperatures and pH, with a half-life of 24 h at 80 °C and retains activity and solubility even after 30 d in a 20% (w/v) K2CO3/pH 11.5 solution─a standard medium for HPC. In pressurized batch reactions, CA-KR1 enhanced CO2 absorption by >90% at 90 °C, 20% K2CO3, and 7 bar. To our knowledge, CA-KR1 constitutes the most resilient CA biocatalyst for efficient CO2 capture under HPC-relevant conditions, reported to date. CA-KR1 integration into industrial settings holds great promise in promoting efficient BioHPC, a potentially game-changing development for enhancing carbon-capture capacity toward industrial decarbonization.
Τίτλος πηγής δημοσίευσης:	Environmental science & technology
Θεματική Κατηγορία:	[EL] Βιοτεχνολογία[EN] Biotechnology [EL] Βιοχημεία[EN] Biochemistry
Λέξεις-Κλειδιά:	BioHPC carbon capture carbonic anhydrases industrial biotechnology metagenomics
Κάτοχος πνευματικών δικαιωμάτων:	© 2024 The Authors. Published byAmerican Chemical Society
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1021/acs.est.4c04291
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο