Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Delineating organizational principles of the endogenous L-A virus by cryo-EM and computational analysis of native cell extracts
Δημιουργός/Συγγραφέας:	Schmidt, Lisa Tüting, Christian Kyrilis, Fotis L Hamdi, Farzad Semchonok, Dmitry A Hause, Gerd Meister, Annette Ihling, Christian Stubbs, Milton T Sinz, Andrea [EL] Καστρίτης, Παναγιώτης[EN] Kastritis, Panagiotis
Ημερομηνία:	2024-05-10
Γλώσσα:	Αγγλικά
ISSN:	2399-3642
DOI:	10.1038/s42003-024-06204-7
Άλλο:	38730276
Περίληψη:	The high abundance of most viruses in infected host cells benefits their structural characterization. However, endogenous viruses are present in low copy numbers and are therefore challenging to investigate. Here, we retrieve cell extracts enriched with an endogenous virus, the yeast L-A virus. The determined cryo-EM structure discloses capsid-stabilizing cation-π stacking, widespread across viruses and within the Totiviridae, and an interplay of non-covalent interactions from ten distinct capsomere interfaces. The capsid-embedded mRNA decapping active site trench is supported by a constricting movement of two flexible opposite-facing loops. tRNA-loaded polysomes and other biomacromolecules, presumably mRNA, are found in virus proximity within the cell extract. Mature viruses participate in larger viral communities resembling their rare in-cell equivalents in terms of size, composition, and inter-virus distances. Our results collectively describe a 3D-architecture of a viral milieu, opening the door to cell-extract-based high-resolution structural virology.
Τίτλος πηγής δημοσίευσης:	Communications biology
Τόμος/Κεφάλαιο:	7
Τεύχος:	1
Θεματική Κατηγορία:	[EL] Δομική Βιολογία[EN] Structural Biology [EL] Ιολογία[EN] Virology [EL] Εφαρμοσμένη οπτική. Φωτονική[EN] Applied optics. Photonics
Λέξεις-Κλειδιά:	Capsid Cell Extracts Saccharomyces cerevisiae RNA, Viral RNA, Messenger Cryoelectron Microscopy
Όροι και προϋποθέσεις δικαιωμάτων:	This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.1016/j.optlastec.2024.111286
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο