Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	AP-1 recognizes sequence elements on HIV-1 LTR in human epithelial tumor cell lines
Δημιουργός/Συγγραφέας:	[EL] Ζουμπουρλής, Βασίλης[EN] Zoumpourlis, Vassilis Ergazaki, M. [EL] Σπαντίδος, Δημήτριος Α.[EN] Spandidos, Demetrios A.
Εκδότης:	Demetrios A. Spandidos Ed. & Pub.
Ημερομηνία:	1994
Γλώσσα:	Αγγλικά
ISSN:	1021-335X
Περίληψη:	Investigation of the nucleotide sequence of the HIV-1 LTR showed the presence of four novel short DNA regions which are homologous to the recognition site for the cellular transcription factor AP-1. Four short oligonucleotide hybrids containing these potential AP-1 sites were constructed and used in gel retardation assays and in competition experiments in order to determine the role of the AP-1 protein in the regulation of HIV-1 expression. The breast MDA MB 468 and cervical HeLa tumor cell lines, which are known to overexpress the AP-1 protein were used in a gel retardation assay as a control to study the affinity of the AP-1 to synthesized oligonucleotide sequences. We have observed specific binding of nuclear factor AP-1 to three of these oligonucleotide hybrids. These results demonstrate the presence of three novel AP-1 binding sites on HIV-1 LTR, one of which was found within the TAR element and in the Tat protein binding region. Moreover, they suggest that AP-1 could be contributing to HIV-1 transcriptional regulation through its interaction with the AP-1 binding sites of HIV-1 LTR.
Τίτλος πηγής δημοσίευσης:	Oncology Reports
Τόμος/Κεφάλαιο:	1
Τεύχος:	2
Σελίδες:	397-401
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 1994 Spandidos Publications Ltd. "Reproduced by permission of the Spandidos Publications Ltd"
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ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο