Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Detection of K-ras mutations in non-small cell lung carcinoma
Δημιουργός/Συγγραφέας:	Neville, E. M. Ellison, G. Kiaris, H. Stewart, M. [EL] Σπαντίδος, Δημήτριος Α.[EN] Spandidos, Demetrios A. Fox, J. C. Field, J. K.
Εκδότης:	Demetrios A. Spandidos Ed. & Pub.
Ημερομηνία:	1995
Γλώσσα:	Αγγλικά
ISSN:	1019-6439
Περίληψη:	Forty-five non-small cell lung cancers (NSCLC) were examined for the presence of K-ras mutations in codon 12 using RFLP (restriction fragment length polymorphism) and ARMS (amplification refractory mutation system) assays. The RFLP analysis consisted of a PCR and subsequent digestion of the product with BstNI. Three adenocarcinomas and one adenosquamous carcinoma were shown to have mutations at codon 12. All of these samples were also examined using the ARMS assay for mutations at codon 12 and second base G to A transitions at codon 13 of the K-ras gene. The same four samples were confirmed to have a single base change in codon 12. No G to A transitions were found at codon 13. The four mutations were: one G to C transversion, one G to A transition and two G to Τ transversions. All mutations occurred at the second position of codon 12 as shown by the ARMS assay. Both of these techniques are rapid and reproducible for the identification of mutations in the K-ras gene and have potential for use in cancer diagnosis.
Τίτλος πηγής δημοσίευσης:	International Journal of Oncology
Τόμος/Κεφάλαιο:	7
Τεύχος:	3
Σελίδες:	511-514
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 1995 Spandidos Publications Ltd. "Reproduced by permission of the Spandidos Publications Ltd"
Όροι και προϋποθέσεις δικαιωμάτων:	By accessing and/or using this content , you agree to be bound by Spandidos Publications Terms and Conditions (www.spandidos-publications.com). It is permitted to view, print or download an insubstantial amount of for your own personal non-commercial use.
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www.spandidos-publications.com/ijo/
ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο