Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Detection of hepatitis Β virus DNA at high frequency in liver neoplasias using a PCR technique
Δημιουργός/Συγγραφέας:	Haliassos, A. Arvanitis, D. Parliaras, J. [EL] Σπαντίδος, Δημήτριος Α.[EN] Spandidos, Demetrios A.
Εκδότης:	Demetrios A. Spandidos Ed. & Pub.
Ημερομηνία:	1992
Γλώσσα:	Αγγλικά
ISSN:	1019-6439
Περίληψη:	Hepatocellular carcinoma is the most frequent liver cancer and hepatitis Β virus is included among the risk factors for the development of this type of neoplasia. Direct detection of this virus is difficult due to the lack of a simple tissue culture system for growing the virus. Amplification of HBV nucleic acid sequences with the Polymerase Chain Reaction technique leads to the direct detection of the virus, but involves several critical steps and it is prone to false positive results due to inter sample contaminations. We overcame these shortcomings by using a simple boiling method for extracting DNA from histological slides of tissues coupled with double ('nested') PCR amplification. In this study we evaluated the frequency of presence of HBV nucleic acid sequences in samples of neoplastic liver tissues from patients in Greece. We studied 20 DNA samples from hepatocellular carcinomas and we found 11 positive for HBV DNA and 8 DNA samples from hepatoblastomas and we found 3 positive for the viral DNA.
Τίτλος πηγής δημοσίευσης:	International Journal of Oncology
Τόμος/Κεφάλαιο:	1
Τεύχος:	2
Σελίδες:	125-128
Αξιολόγηση από ομότιμους (peer reviewed):	Ναι
Κάτοχος πνευματικών δικαιωμάτων:	© 1993 Spandidos Publications Ltd. "Reproduced by permission of the Spandidos Publications Ltd"
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ΙΒΦΧΒ: αρχειακή συλλογή:	Ινστιτούτο Βιολογικών Ερευνών και Βιοτεχνολογίας (ΙΒΕΒ) (έως 2012)
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο