Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	cPHB biosynthesis regulation by the AtoS-AtoC two-component system in Delta atoDAEB E-coli
Δημιουργός/Συγγραφέας:	Theodorou, E. Theodorou, M. C. [EL] Κυριακίδης, Δ.Α.[EN] Kyriakidis, D.A.
Εκδότης:	Wiley-Blackwell Publishing Ltd.
Ημερομηνία:	2008
Γλώσσα:	Αγγλικά
ISSN:	1742-464X
DOI:	10.1111/j.1742-4658.2008.06448.x
Περίληψη:	The AtoS-AtoC two-component system in E. coli consists of the membrane bound AtoS sensor kinase and the AtoC response regulator. Upon acetoacetate-mediated induction, AtoS autophosporylates and consequently phosphorylates, thereby activating, AtoC. AtoC regulates positively the transcriptional activation of atoDAEB operon, encoding for enzymes involved in short-chain fatty acid catabolism. AtoS-AtoC system acting on its target atoDAEB operon regulates the biosynthesis and the intracellular distribution of short-chain poly-(R)-3-hydroxybutyrate (cPHB), a biopolymer with many physiological roles in E. coli, under acetoacetate or spermidine induction. The extrachromosomal introduction of the atoSC locus and the atoDAEB operon in Dato- DAEB strain increases cPHB levels in the presence of the inducers. Enhancement of cPHB is also achieved by the AtoS-AtoC system in the absence of its target operon. DatoDAEB cells overproducing the AtoS kinase accumulate only the basal cPHB levels independently to the presence of acetoacetate or spermidine. However, AtoC up-regulates cPHB in the absence of atoDAEB operon upon induction. Exposure of DatoDAEB cells which overproduce the AtoS-AtoC system or only AtoC to cerulenin or acrylic acid, results to reduction of cPHB levels. Cerulenin, the inhibitor of fatty acids biosynthesis pathway, caused the more pronounced down-regulation of cPHB. These data suggest that in the absence of atoDAEB operon, AtoC possibly regulates cPHB biosynthesis through the fatty acid metabolism pathways, with the biosynthesis being the most potent mechanism. This work was supported by doctoral research scholarships of the ‘State Scholarships Foundation’ and ‘Onassis Foundation’.
Τίτλος πηγής δημοσίευσης:	The FEBS Journal
Τόμος/Κεφάλαιο:	275
Τεύχος:	s 1
Σελίδες:	290-290
Κάτοχος πνευματικών δικαιωμάτων:	© 2008 The Authors Journal compilation © 2008 FEBS
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www3.interscience.wiley.com/journal/119877016/tocgroup
Σημειώσεις:	Abstract of Poster Preseantation
Εμφανίζεται στις συλλογές:	Άλλα