Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Interactions of the antizyme AtoC with regulatory elements of the Escherichia coli atoDAEB operon
Δημιουργός/Συγγραφέας:	Matta, M.K. Lioliou, E.E. Panagiotidis, C.H. [EL] Κυριακίδης, Δ.Α.[EN] Kyriakidis, D.A. Panagiotidis, C.A.
Εκδότης:	American Society for Microbiology
Ημερομηνία:	2007-09
Γλώσσα:	Αγγλικά
ISSN:	0021-9193
DOI:	10.1128/jb.00214-07
Περίληψη:	AtoC has a dual function as both an antizyme, the posttranslational inhibitor of polyamine biosynthetic enzymes, and the transcriptional regulator of genes involved in short-chain fatty acid catabolism (the atoDAEB operon). We have previously shown that AtoC is the response egulator of the AtoS-AtoC two-component signal transduction system that activates atoDAEB when Escherichia coli is exposed to acetoacetate. Here, we show that the same cis elements control both promoter inducibility and AtoC binding. Chromatin immunoprecipitation experiments confirmed the acetoacetate-inducible binding of AtoC to the predicted DNA region in vivo. DNase I protection footprinting analysis revealed that AtoC binds two 20-bp stretches, constituting an inverted palindrome, that are located at 146 to 107 relative to the transcription initiation site. Analyses of promoter mutants obtained by in vitro chemical mutagenesis of the atoDAEB promoter verified both the importance of AtoC binding for the inducibility of the promoter by acetoacetate and the 54 dependence of atoDAEB expression. The integration host factor was also identified as a critical component of the AtoC-mediated induction of atoDAEB.
Τίτλος πηγής δημοσίευσης:	Journal of Bacteriology
Τόμος/Κεφάλαιο:	189
Τεύχος:	17
Σελίδες:	6324-6332
Κάτοχος πνευματικών δικαιωμάτων:	Copyright © 2007, American Society for Microbiology
Ηλεκτρονική διεύθυνση με ανοικτή πρόσβαση (link):	http://www.ncbi.nlm.nih.gov/sites/entrez
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://jb.asm.org/
Σημειώσεις:	This is also freely available in PubMed Central at: http://www.ncbi.nlm.nih.gov/sites/entrez
Εμφανίζεται στις συλλογές:	Άλλα