Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Molecular modeling and functional analysis of the AtoS–AtoC two-component signal transduction system of Escherichia coli
Δημιουργός/Συγγραφέας:	Grigoroudisa, A.I. Panagiotidis, C.A. Liolioua, E.E. Vlassic, M. [EL] Κυριακίδης, Δ.Α.[EN] Kyriakidis, D.A.
Εκδότης:	Elsevier B.V.
Ημερομηνία:	2007
Γλώσσα:	Αγγλικά
ISSN:	0304-4165
DOI:	10.1016/j.bbagen.2007.04.004
Περίληψη:	The AtoS-AtoC two-component signal transduction system positively regulates the expression of the atoDAEB operon in Escherichia coli. Upon acetoacetate induction, AtoS sensor kinase autophosphorylates and subsequently phosphorylates, thereby activating, the response regulator AtoC. In a previous work we have shown that AtoC is phosphorylated at both aspartate 55 and histidine73. In this study, based on known three-dimensional structures of other two component regulatory systems, we modeled the 3D-structure of the receiver domain of AtoC in complex with the putative dimerization/autophosphorylation domain of the AtoS sensor kinase. The produced structural model indicated that aspartate 55, but not histidine 73, of AtoC is in close proximity to the conserved, putative phosphate-donor, histidine (H398) of AtoS suggesting that aspartate 55 may be directly involved in the AtoS-AtoC phosphate transfer. Subsequent biochemical studies with purified recombinant proteins showed that AtoC mutants with alterations of aspartate 55, but not histidine 73, were unable to participate in the AtoS-AtoC phosphate transfer in support of the modeling prediction. In addition, these AtoC mutants displayed reduced DNA-dependent ATPase activity, although their ability to bind their target DNA sequences in a sequence-specific manner was found to be unaltered. © 2007 Elsevier B.V. All rights reserved.
Τίτλος πηγής δημοσίευσης:	Biochimica et Biophysica Acta -General Subjects
Τόμος/Κεφάλαιο:	1770
Τεύχος:	8
Σελίδες:	1248-1258
Λέξεις-Κλειδιά:	AtoS AtoC Two-component system Antizyme ATPase DNA-binding σ54-regulator factor Structural model
Κάτοχος πνευματικών δικαιωμάτων:	© 2007 Elsevier B.V.
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www.elsevier.com/locate/bbagen
Εμφανίζεται στις συλλογές:	Άλλα