Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Flagellin is co-excreted with the PHB depolymerase in Thermus thermophilus growth media
Δημιουργός/Συγγραφέας:	Papaneophytou, C. P. Pantazaki, A. A. Hirano, H. [EL] Κυριακίδης, Δ.Α.[EN] Kyriakidis, D.A.
Εκδότης:	Wiley-Blackwell Publishing Ltd.
Ημερομηνία:	2008
Γλώσσα:	Αγγλικά
ISSN:	1742-464X
DOI:	10.1111/j.1742-4658.2008.06448.x
Περίληψη:	T. thermophilus HB8 (DSM Acc No 579) was grown in minimal salt medium (MSM) containing sodium gluconate as carbon source [1]. PHA depolymerase activity was assayed using PHB as substrate [2]. Flagellin was concentrated from cell free supernatant by precipitation with trichloroacetic acid. Purification of flagella was performed as described previously [3]. Immunoblots were carried out using flagellin antibody (anti-FliC; Salmonella H antiserum rabbit). During the growth of T. thermophilus in MSM progressive increase of PHB depolymerase and flagellin was obtained. Analysis by SDS-PAGE and western blot of purified flagella revealed a single band of 62 kDa. Two stable flagellin fragments of 51 and 32 kDa were observed after incubation at 100_C for 20 minutes or after acid pH treatment, respectively. Flagellin was isolated in a complex form with PHB-depolymerase either by precipitation with PHB or by co-immunoprecibitation with protein-A sepharose/anti-FliC. Furthermore, the fragment of 32 kDa was characterized as flagellin by N-terminal and intenal sequence analysis. The extracellular PHB depolymerase of T. thermophilus forms complex with flagellin of the type III export system. These results indicate that T. thermophilus HB8 is a motile bacterium, capable of sensing environmental changes or escaping from adverse conditions
Τίτλος πηγής δημοσίευσης:	The FEBS Journal
Τόμος/Κεφάλαιο:	275
Τεύχος:	s 1
Σελίδες:	422-422
Κάτοχος πνευματικών δικαιωμάτων:	© 2008 The Authors Journal compilation © 2008 FEBS
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www3.interscience.wiley.com/journal/119877016/tocgroup
Σημειώσεις:	Abstract Poster Presentation
Εμφανίζεται στις συλλογές:	Άλλες δράσεις