Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Heterologous expression of a hyperthermophilic alpha-amylase in xanthan gum producing Xanthomonas campestris cells
Δημιουργός/Συγγραφέας:	Konsoula, Z. Liakopoulou-Kyriakides, M. Perysinakis, A. Chira, P. Afendra, A. Drainas, C. [EL] Κυριακίδης, Δ.Α.[EN] Kyriakidis, D.A.
Εκδότης:	Humana Press, Inc.
Ημερομηνία:	2008
Γλώσσα:	Αγγλικά
ISSN:	0273-2289
DOI:	10.1007/s12010-007-8115-x
Περίληψη:	A hyperthermophilic α-amylase encoding gene from Pyrococcus woesei was transferred and expressed in Xanthomonas campestris ATCC 13951. The heterologous α-amylase activity was detected in the intracellular fraction of X. campestris and presented similar thermostability and catalytic properties with the native P. woesei enzyme. The recombinant α-amylase was found to be stable at 90 °C for 4 h and within the same period it retained more than 50% of its initial activity at 110 °C. Furthermore, X. campestris transformants produced similar levels of recombinant α-amylase activity regardless of the carbon source present in the growth medium, whereas the native X. campestris α-amylase production was highly dependent on starch availability and it was suppressed in the presence of glucose or other reducing sugars. On the other hand, xanthan gum yield, which appeared to be similar for both wild type and recombinant X. campestris strains, was enhanced at higher starch or glucose concentrations. Evidence presented in this study supports that X. campestris is a promising cell factory for the co-production of recombinant hyperthermophilic α-amylase and xanthan gum. © 2007 Humana Press Inc.
Τίτλος πηγής δημοσίευσης:	Applied Biochemistry and Biotechnology
Τόμος/Κεφάλαιο:	149
Τεύχος:	2
Σελίδες:	99-108
Λέξεις-Κλειδιά:	Hyperthermophilicity α-amylase Xanthan gum Xanthomonas campestris
Κάτοχος πνευματικών δικαιωμάτων:	© Humana Press Inc. 2007
Ηλεκτρονική διεύθυνση περιοδικού (link) :	http://www.springerlink.com/content/120548/
Εμφανίζεται στις συλλογές:	Άλλα