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https://hdl.handle.net/10442/8338
| Εξειδίκευση τύπου : | Άρθρο σε επιστημονικό περιοδικό |
| Τίτλος: | Extracellular Ca2+ transients affect poly-(R)-3-hydroxybutyrate regulation by the AtoS-AtoC system in Escherichia coli |
| Δημιουργός/Συγγραφέας: | Theodorou, Marina C.
Tiligada, Ekaterini
[EL] Κυριακίδης, Δ.Α.[EN] Kyriakidis, D.A. |
| Εκδότης: | Portland Press, Great Britain |
| Ημερομηνία: | 2009-02-01 |
| Γλώσσα: | Αγγλικά |
| ISSN: | 0264-6021 |
| DOI: | 10.1042/bj20081169 |
| Περίληψη: | Escherichia coli is exposed to wide extracellular concentrations of Ca2+, whereas the cytosolic levels of the ion are subject to stringent control and are implicated in many physiological functions. The present study shows that extracellularCa2+ controls cPHB [complexed poly-(R)-3-hydroxybutyrate] biosynthesis through the AtoS-AtoC two-component system. Maximal cPHB accumulation was observed at higher [Ca2+]e (extracellular Ca2+ concentration) in AtoS-AtoC-expressing E. coli compared with their _atoSC counterparts, in both cytosolic and membrane fractions. The reversal of EGTA-mediated down-regulation of cPHB biosynthesis by the addition of Ca2+ and Mg2+ was under the control of the AtoS-AtoC system.Moreover, the Ca2+-channel blocker verapamil reduced total and membrane-bound cPHB levels, the inhibitory effect being circumvented by Ca2+ addition only in atoSC+ bacteria. Histamine and compound 48/80 affected cPHB accumulation in a [Ca2+]e-dependent manner directed by theAtoS-AtoC system. In conclusion, these data provide evidence for the involvement of external Ca2+ on cPHB synthesis regulated by the AtoS-AtoC two-component system, thus linking Ca2+ with a signal transduction system, most probably through a transporter. Key words: AtoS-AtoC two-component system, atoDAEB, calcium, calcium channel blocker, histamine, poly-(R)-3- hydroxybutyrate. |
| Τίτλος πηγής δημοσίευσης: | Biochemical Journal |
| Τόμος/Κεφάλαιο: | 417 |
| Σελίδες: | 667–672 |
| Θεματική Κατηγορία: | [EL] Χημεία (Γενικά)[EN] Chemistry (General)
[EL] Βιολογία (Γενικά)[EN] Biology (General) |
| Λέξεις-Κλειδιά: | Escherichia coli
AtoS-AtoC system
cPHB
extracellular Ca2 |
| Κάτοχος πνευματικών δικαιωμάτων: | © The Authors Journal compilation© 2009 Biochemical Society |
| Ηλεκτρονική διεύθυνση περιοδικού (link) : | http://www.biochemj.org/bj/default.htm |
| Σημειώσεις: | This research was funded by the project 26515 “BIOPRODUCTION” of the 6th Framework Programme (FP6) of the European Union. |
| Εμφανίζεται στις συλλογές: | Άλλες δράσεις
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