Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Aqueous Heat Method for the Preparation of Hybrid Lipid-Polymer Structures: From Preformulation Studies to Protein Delivery
Δημιουργός/Συγγραφέας:	Pippa, Natassa Lagopati, Nefeli Forys, Aleksander Chountoulesi, Maria Katifelis, Hektor Chrysostomou, Varvara Trzebicka, Barbara Gazouli, Maria Demetzos, Costas [EL] Πίσπας, Στέργιος[EN] Pispas, Stergios
Ημερομηνία:	2022-05-24
Γλώσσα:	Αγγλικά
ISSN:	2227-9059
DOI:	10.3390/biomedicines10061228
Άλλο:	35740250
Περίληψη:	Liposomes with adjuvant properties are utilized to carry biomolecules, such as proteins, that are often sensitive to the stressful conditions of liposomal preparation processes. The aim of the present study is to use the aqueous heat method for the preparation of polymer-grafted hybrid liposomes without any additional technique for size reduction. Towards this scope, liposomes were prepared through the combination of two different lipids with adjuvant properties, namely dimethyldioctadecylammonium (DDA) and D-(+)-trehalose 6,6'-dibehenate (TDB) and the amphiphilic block copolymer poly(2-(dimethylamino)ethyl methacrylate)-b-poly(lauryl methacrylate) (PLMA-b-PDMAEMA). For comparison purposes, PAMAM dendrimer generation 4 (PAMAM G4) was also used. Preformulation studies were carried out by differential scanning calorimetry (DSC). The physicochemical characteristics of the prepared hybrid liposomes were evaluated by light scattering and their morphology was evaluated by cryo-TEM. Subsequently, in vitro nanotoxicity studies were performed. Protein-loading studies with bovine serum albumin were carried out to evaluate their encapsulation efficiency. According to the results, PDMAEMA-b-PLMA was successfully incorporated in the lipid bilayer, providing improved physicochemical and morphological characteristics and the ability to carry higher cargos of protein, compared to pure DDA:TDB liposomes, without affecting the biocompatibility profile. In conclusion, the aqueous heat method can be applied in polymer-grafted hybrid liposomes for protein delivery without further size-reduction processes.
Τίτλος πηγής δημοσίευσης:	Biomedicines
Τόμος/Κεφάλαιο:	10
Τεύχος:	6
Θεματική Κατηγορία:	[EL] Φυσική και θεωρητική χημεία[EN] Physical and theoretical chemistry [EL] Νανοτεχνολογία[EN] Nanotechnology
Λέξεις-Κλειδιά:	block copolymer bovine serum albumin cationic lipids cryogenic transmission electron microscopy (cryo-TEM) differential scanning calorimetry (DSC)
EU Grant:	Reinforcement of Postdoctoral Researchers—2nd Cycle
EU Grant identifier:	MIS-5033021
Κάτοχος πνευματικών δικαιωμάτων:	© 2022 by the authors. Licensee MDPI, Basel, Switzerland
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://www.mdpi.com/2227-9059/10/6/1228
Σημειώσεις:	supporting information: https://www.mdpi.com/article/10.3390/biomedicines10061228/s1
Εμφανίζεται στις συλλογές:	Ινστιτούτο Θεωρητικής και Φυσικής Χημείας (ΙΘΦΧ) - Επιστημονικό έργο