Εξειδίκευση τύπου :	Άρθρο σε επιστημονικό περιοδικό
Τίτλος:	Bacterial Lectin FimH and Its Aggregation Hot-Spots: An Alternative Strategy against Uropathogenic Escherichia coli
Δημιουργός/Συγγραφέας:	Nasi, Georgia I Georgakopoulou, Konstantina I Theodoropoulou, Marilena K Papandreou, Nikos C [EL] Χρυσίνα, Ευαγγελία Δ.[EN] Chrysina, Evangelia D. Tsiolaki, Paraskevi L Iconomidou, Vassiliki A
Ημερομηνία:	2023-03-22
Γλώσσα:	Αγγλικά
ISSN:	1999-4923
DOI:	10.3390/pharmaceutics15031018
Άλλο:	36986878
Περίληψη:	Type I fimbriae are the main adhesive organelles of uropathogenic Escherichia coli (UPEC), consisting of four different subunits. Their component with the most important role in establishing bacterial infections is the FimH adhesin located at the fimbrial tip. This two-domain protein mediates adhesion to host epithelial cells through interaction with terminal mannoses on epithelial glycoproteins. Here, we propose that the amyloidogenic potential of FimH can be exploited for the development of therapeutic agents against Urinary Tract Infections (UTIs). Aggregation-prone regions (APRs) were identified via computational methods, and peptide-analogues corresponding to FimH lectin domain APRs were chemically synthesized and studied with the aid of both biophysical experimental techniques and molecular dynamic simulations. Our findings indicate that these peptide-analogues offer a promising set of antimicrobial candidate molecules since they can either interfere with the folding process of FimH or compete for the mannose-binding pocket.
Τίτλος πηγής δημοσίευσης:	Pharmaceutics
Τόμος/Κεφάλαιο:	15
Τεύχος:	3
Θεματική Κατηγορία:	[EL] Θεραπευτική. Φαρμακολογία[EN] Therapeutics.Pharmacology [EL] Δομική Βιολογία[EN] Structural Biology [EL] Φασματοσκοπία[EN] Spectroscopy [EL] Βιοπληροφορική[EN] Bioinformatics
Λέξεις-Κλειδιά:	FimH UPEC UTIs aggregation-prone regions lectin prediction type I fimbriae
EU Grant:	INSPIRED-The National Research Infrastructures on Integrated Structural Biology, Drug Screening Efforts and Drug target functional characterization Strengthening Human Resources Research Potential via Doctorate Research—2nd Cycle
EU Grant identifier:	MIS 5002550 MIS-5000432 PR010018—FimbriaeDynamics
Κάτοχος πνευματικών δικαιωμάτων:	© 2023 by the authors. Licensee MDPI, Basel, Switzerland.
Όροι και προϋποθέσεις δικαιωμάτων:	This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Ηλεκτρονική διεύθυνση στον εκδότη (link):	https://doi.org/10.3390/pharmaceutics15031018
Εμφανίζεται στις συλλογές:	Ινστιτούτο Χημικής Βιολογίας - Επιστημονικό έργο